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Literature summary extracted from
- Cloning and expression of recombinant human pineal tryptophan hydroxylase in Escherichia coli: purification and characterization of the cloned enzyme (1999), Biochim. Biophys. Acta, 1434, 317-330.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.16.4 | expression in Escherichia coli | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.4 | (7R)-5,6,7,8-tetrahydrobiopterin | recombinant pinal enzyme, 2.1 mM, 50% inhibition | Homo sapiens |  |
| 1.14.16.4 | L-5-hydroxytryptophan | - |
Homo sapiens | |
| 1.14.16.4 | phenylalanine | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.4 | 0.02 | - |
L-tryptophan | cofactor (6R)-5,6,7,8-tetrahydrobipterin | Homo sapiens | |
| 1.14.16.4 | 0.023 | - |
L-tryptophan | cofactor 6-methyl-5,6,7,8-tetrahydrobipterin | Homo sapiens | |
| 1.14.16.4 | 0.045 | - |
(6R)-5,6,7,8-tetrahydrobiopterin | substrate tryptophan | Homo sapiens | |
| 1.14.16.4 | 0.075 | - |
6-methyl-5,6,7,8-tetrahydrobiopterin | substrate tryptophan | Homo sapiens | |
| 1.14.16.4 | 0.09 | - |
L-phenylalanine | cofactor (6R)-5,6,7,8-tetrahydrobipterin | Homo sapiens | |
| 1.14.16.4 | 2.1 | - |
(7R)-5,6,7,8-tetrahydrobiopterin | substrate tryptophan | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.4 | Fe2+ | recombinant pineal enzyme contains 0.2-0.3 mol of iron/mol of enzyme | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 50000 | - |
4 * 50000, recombinant pineal enzyme, SDS-PAGE | Homo sapiens |
| 1.14.16.4 | 200000 | - |
recombinant pineal enzyme, gel filtration | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.16.4 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.16.4 | recombinant pineal enzyme, ammonium sulfate, Pterin-agarose, Sepharose | Homo sapiens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 0.6 | - |
recombinant pineal enzyme | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.4 | L-phenylalanine + (7R)-5,6,7,8-tetrahydrobiopterine + O2 | - |
Homo sapiens | L-tyrosine + dihydropteridine + H2O | - |
r | |
| 1.14.16.4 | L-tryptophan + 6-methyl-tetrahydropterin + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + 6-methyl-4a-hydroxytetrahydropterin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.16.4 | tetramer | 4 * 50000, recombinant pineal enzyme, SDS-PAGE | Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.4 | 0.035 | - |
L-5-hydroxytryptophan | - |
Homo sapiens | |
| 1.14.16.4 | 0.15 | - |
phenylalanine | - |
Homo sapiens | |
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