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Literature summary extracted from

  • Hamdan, F.F.; Ribeiro, P.
    Characterization of a stable form of tryptophan hydroxylase from the human parasite Schistosoma mansoni (1999), J. Biol. Chem., 274, 21746-21754.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.4 His-tagged enzyme, expression in Escherichia coli Schistosoma mansoni

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.4 5-hydroxytryptophan 0.018 mM, 50% inhibition Schistosoma mansoni
1.14.16.4 D,L-4-chlorophenylalanine 0.009 mM, 50% inhibition Schistosoma mansoni
1.14.16.4 dopamine 0.022 mM, 50% inhibition Schistosoma mansoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.4 0.0067
-
 tetrahydrobiopterin recombinant His-tagged enzyme Schistosoma mansoni
1.14.16.4 0.022
-
 L-tryptophan recombinant His-tagged enzyme Schistosoma mansoni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.4 60000
-
 4 * 60000, His-tagged recombinant enzyme, immunoblot Schistosoma mansoni
1.14.16.4 240000
-
 recombinant His-tagged enzyme, gel filtration Schistosoma mansoni

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.4 Oryctolagus cuniculus
-
-
-
1.14.16.4 Schistosoma mansoni O96370
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.4 recombinant His-tagged enzyme, Nickel affinity chromatography Schistosoma mansoni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.4 0.17
-
 recombinant His-tagged enzyme Schistosoma mansoni

Storage Stability

EC Number Storage Stability Organism
1.14.16.4 recombinant His-tagged enzyme, -80°C, 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% glycerol, 0.05% Tween 20, 1 mM dithiothreitol, 4 days, no loss of activity Schistosoma mansoni
1.14.16.4 recombinant His-tagged enzyme, 4°C, 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% glycerol, 0.05% Tween 20, 1 mM dithiothreitol, 4 days, no loss of activity Schistosoma mansoni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.4 L-tryptophan + tetrahydrobiopterin + O2
-
 Schistosoma mansoni 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 r
1.14.16.4 L-tryptophan + tetrahydropteridine + O2
-
 Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
1.14.16.4 L-tryptophan + tetrahydropteridine + O2
-
 Schistosoma mansoni 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.4 tetramer
-
 Oryctolagus cuniculus
1.14.16.4 tetramer 4 * 60000, His-tagged recombinant enzyme, immunoblot Schistosoma mansoni

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.16.4 37
-
 half-life: 54 min Oryctolagus cuniculus
1.14.16.4 37
-
 80 min, no loss of activity, estimated half-life: 21 h Schistosoma mansoni