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Literature summary extracted from

  • Moran, G.R.; Daubner, S.C.; Fitzpatrick, P.F.
    Expression and characterization of the catalytic core of tryptophan hydroxylase (1998), J. Biol. Chem., 273, 12259-12266.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.4 expression of wild-type and N- and C-terminal truncated mutant enzyme in Escherichia coli Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.4 0.0058
-
 L-tryptophan recombinant N- and C-terminal truncated enzyme, cofactor 6-methyltetrahydrobipterin Oryctolagus cuniculus
1.14.16.4 0.0142
-
 tetrahydrobiopterin recombinant N- and C-terminal truncated enzyme, substrate phenylalanine Oryctolagus cuniculus
1.14.16.4 0.0146
-
 6-methyltetrahydropterin recombinant N- and C-terminal truncated enzyme, substrate phenylalanine Oryctolagus cuniculus
1.14.16.4 0.0479
-
 L-tryptophan recombinant N- and C-terminal truncated enzyme, cofactor tetrahydrobiopterin Oryctolagus cuniculus
1.14.16.4 0.0606
-
 L-phenylalanine recombinant N- and C-terminal truncated enzyme, cofactor tetrahydrobiopterin Oryctolagus cuniculus
1.14.16.4 0.102
-
 L-phenylalanine recombinant N- and C-terminal truncated enzyme, cofactor 6-methyltetrahydropterin Oryctolagus cuniculus
1.14.16.4 0.135
-
 tetrahydrobiopterin recombinant N- and C-terminal truncated enzyme, substrate tryptophan Oryctolagus cuniculus
1.14.16.4 0.177
-
 6-methyltetrahydropterin recombinant N- and C-terminal truncated enzyme, substrate tryptophan Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.4 Fe2+ N- and C-terminal recombinant enzyme, iron is required for activity Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.4 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.4 recombinant N- and C-terminal truncated mutant enzyme Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.14.16.4 N- and C-terminal truncated recombinant enzyme, -70°C, 100 mM ammonium sulfate, ferrous iron, 10% glycerol, 2 mM dithiothreitiol, pH 7.0, indefinitely, no loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.4 L-phenylalanine + tetrahydropteridine + O2
-
 Oryctolagus cuniculus tyrosine + dihydropteridine + H2O
-
 ?
1.14.16.4 L-tryptophan + tetrahydrobiopterin + O2
-
 Oryctolagus cuniculus 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.4 L-tryptophan + tetrahydropteridine + O2
-
 Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?