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Literature summary extracted from
- Enzymic studies on the biosynthesis of serotonin in mammalian brain (1970), J. Biol. Chem., 245, 1699-1709.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.4 | 0.002 | - |
L-tryptophan | enzyme from crude mitochondrial fraction | Cavia porcellus |  |
| 1.14.16.4 | 0.006 | - |
2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine | soluble enzyme from brain | Cavia porcellus | |
| 1.14.16.4 | 0.3 | - |
L-tryptophan | soluble enzyme from brain | Cavia porcellus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.16.4 | particle-bound | - |
Cavia porcellus | - |
- |
| 1.14.16.4 | particle-bound | - |
Bos taurus | - |
- |
| 1.14.16.4 | soluble | - |
Cavia porcellus | - |
- |
| 1.14.16.4 | soluble | - |
Bos taurus | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.4 | L-tryptophan + tetrahydropteridine + O2 | Cavia porcellus | enzyme catalyzes the rate-limiting step in biosynthesis of putative neurotransmitter 5-hydroxytryptamine, serotonin | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
| 1.14.16.4 | L-tryptophan + tetrahydropteridine + O2 | Bos taurus | enzyme catalyzes the rate-limiting step in biosynthesis of putative neurotransmitter 5-hydroxytryptamine, serotonin | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.16.4 | Bos taurus | - |
- |
- |
| 1.14.16.4 | Cavia porcellus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.16.4 | - |
Cavia porcellus |
| 1.14.16.4 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.16.4 | brain | serotonergic neurons | Cavia porcellus | - |
| 1.14.16.4 | brain | serotonergic neurons | Bos taurus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.16.4 | -20°C, 2 months, 40% loss of activity | Cavia porcellus |
| 1.14.16.4 | -20°C, 2 months, 40% loss of activity | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.4 | L-tryptophan + 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine + O2 | - |
Cavia porcellus | ? | - |
r | |
| 1.14.16.4 | L-tryptophan + tetrahydropteridine + O2 | - |
Cavia porcellus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
| 1.14.16.4 | L-tryptophan + tetrahydropteridine + O2 | - |
Bos taurus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
| 1.14.16.4 | L-tryptophan + tetrahydropteridine + O2 | enzyme catalyzes the rate-limiting step in biosynthesis of putative neurotransmitter 5-hydroxytryptamine, serotonin | Cavia porcellus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
| 1.14.16.4 | L-tryptophan + tetrahydropteridine + O2 | enzyme catalyzes the rate-limiting step in biosynthesis of putative neurotransmitter 5-hydroxytryptamine, serotonin | Bos taurus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 8 | 8.5 | soluble enzyme from brain | Cavia porcellus |
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Release 2023.1 (January 2023)
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