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Literature summary extracted from

  • Friedman, P.A.; Kappelman, A.H.; Kaufman, S.
    Partial purification and characterization of tryptophan hydroxylase from rabbit hindbrain (1972), J. Biol. Chem., 247, 4165-4173.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.16.4 Fe2+ protects against inactivation by H2O2 in absence of catalase Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.4 H2O2 complete dependence on catalase in the absence of a mercaptan Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.4 additional information
-
 additional information 20% O2, cofactor 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine, 2.5% O2, cofactor tetrahydrobipterine Oryctolagus cuniculus
1.14.16.4 0.031
-
 tetrahydrobiopterin
-
 Oryctolagus cuniculus
1.14.16.4 0.05
-
 tryptophan cofactor tetrahydrobiopterine Oryctolagus cuniculus
1.14.16.4 0.078
-
 tryptophan cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Oryctolagus cuniculus
1.14.16.4 0.13
-
 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine
-
 Oryctolagus cuniculus
1.14.16.4 0.29
-
 tryptophan cofactor 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.4 Fe2+ stimulates in the absence of catalase Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.4 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.4 partial Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.4 brain hindbrain Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.4 0.00018
-
 partially purified enzyme from hindbrain Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.14.16.4 -80°C, N2 atmosphere, dithiothreitol, 2 weeks, 20% loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.4 L-tryptophan + 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine + O2
-
 Oryctolagus cuniculus ?
-
 r
1.14.16.4 L-tryptophan + tetrahydrobiopterin + O2
-
 Oryctolagus cuniculus 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.4 L-tryptophan + tetrahydropteridine + O2 cofactor tetrahydrobiopterin Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
1.14.16.4 L-tryptophan + tetrahydropteridine + O2 cofactor 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
1.14.16.4 L-tryptophan + tetrahydropteridine + O2 cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?