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Literature summary extracted from

  • Francisco, W.A.; Merkler, D.J.; Blackburn, N.J.; Klinman, J.P.
    Kinetic mechanism and intrinsic isotope effects for the peptidylglycine alpha-amidating enzyme reaction (1998), Biochemistry, 37, 8244-8252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.3 medullary thyroid alpha-AE, type A, expression in chinese hamster ovary cells Rattus norvegicus
4.3.2.5
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.17.3 extracellular recombinant enzyme secreted into medium Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+ required for alpha-hydroxylation step Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Rattus norvegicus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Rattus norvegicus
-
 bifunctional alpha-AE
-
4.3.2.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.3 type A alpha-AE, recombinant from chinese hamster ovary cells Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.17.3 [peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O sequential activity of 2 enzymes contained within a bifunctional protein Rattus norvegicus
a
1.14.17.3 [peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O equilibrium ordered mechanism Rattus norvegicus
a
1.14.17.3 [peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O kinetic analysis Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 medullary thyroid carcinoma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 hippuric acid + ascorbate + O2 i.e. N-benzoylglycine Rattus norvegicus alpha-hydroxyhippuric acid + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2
-
 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 requirement for O2 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.17.3 More the 2-step process is catalyzed by only 1 enzyme called type A peptidylglycine alpha-amidating enzyme Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate dependent on Rattus norvegicus