Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jaron, S.; Blackburn, N.J.
    Characterization of a half-apo derivative of peptidylglycine monooxygenase. Insight into the reactivity of each active site copper (2001), Biochemistry, 40, 6867-6875.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.3 medullary thyroid alpha-AE, type A, expression in chinese hamster ovary cells Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+ Cu2+ to protein ration is 0.97 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Rattus norvegicus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Rattus norvegicus
-
 bifunctional alpha-AE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.3 type A alpha-AE, recombinant from chinese hamster ovary cells Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.17.3 [peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O structural analysis Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 medullary thyroid carcinoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.3 additional information
-
-
 Rattus norvegicus
1.14.17.3 5.9
-
 purified recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2
-
 Rattus norvegicus alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 dansyl-D-Tyr-Val-Gly + ascorbate + O2
-
 Rattus norvegicus dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2
-
 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 requirement for O2 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.17.3 More the 2-step process is catalyzed by only 1 enzyme called type A peptidylglycine alpha-amidating enzyme Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate
-
 Rattus norvegicus