EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.17.3 | acetyl-D-Leu-OCH2COOH | - |
Bos taurus | |
1.14.17.3 | acetyl-D-Phe-Gly | - |
Bos taurus | |
1.14.17.3 | acetyl-D-Phe-OCH2COOH | - |
Bos taurus | |
1.14.17.3 | acetyl-DL-Phe-OCH2COOH | - |
Bos taurus | |
1.14.17.3 | acetyl-Gly-OCH2COOH | - |
Bos taurus | |
1.14.17.3 | acetyl-L-Leu-OCH2COOH | - |
Bos taurus | |
1.14.17.3 | acetyl-L-Phe-D-Phe-Gly | - |
Bos taurus | |
1.14.17.3 | acetyl-L-Phe-Gly | only substrate for alpha-hydroxylation | Bos taurus | |
1.14.17.3 | acetyl-L-Phe-OCH2COOH | - |
Bos taurus | |
4.3.2.5 | (R)-2-phenylbutyl-alpha-hydroxy-Gly | - |
Bos taurus | |
4.3.2.5 | (S)-2-phenylbutyl-alpha-hydroxy-Gly | - |
Bos taurus | |
4.3.2.5 | phenylacetyl-alpha-hydroxy-Gly | competitive inhibition | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.17.3 | additional information | - |
additional information | - |
Bos taurus | |
4.3.2.5 | 0.027 | - |
trinitrophenyl-D-Tyr-L-Val-alpha-hydroxy-Gly | 37°C, pH 6.5 | Bos taurus | |
4.3.2.5 | 0.23 | - |
acetyl-L-Phe-alpha-hydroxyglycine | 37°C, pH 6.5 | Bos taurus | |
4.3.2.5 | 0.3 | - |
(R)-2-phenylbutyl-alpha-hydroxy-Gly | 37°C, pH 6.5 | Bos taurus | |
4.3.2.5 | 0.33 | - |
(S)-2-phenylbutyl-alpha-hydroxy-Gly | 37°C, pH 6.5 | Bos taurus | |
4.3.2.5 | 0.46 | - |
N-benzoyl-alpha-hydroxy-Gly | 37°C, pH 6.5 | Bos taurus | |
4.3.2.5 | 0.5 | - |
acetyl-D-Phe-alpha-hydroxyglycine | 37°C, pH 6.5 | Bos taurus | |
4.3.2.5 | 0.56 | - |
(S)-O-acetyl-mandelyl-alpha-hydroxyglycine | 37°C, pH 6.5 | Bos taurus | |
4.3.2.5 | 2.6 | - |
(R)-O-acetyl-mandelyl-alpha-hydroxyglycine | 37°C, pH 6.5 | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.3 | peptidylglycine + ascorbate + O2 | Bos taurus | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.17.3 | Bos taurus | - |
- |
- |
4.3.2.5 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.17.3 | - |
Bos taurus |
4.3.2.5 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.17.3 | pituitary gland | - |
Bos taurus | - |
4.3.2.5 | pituitary gland | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.3 | additional information | EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PMH) and 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase | Bos taurus | ? | - |
? | |
1.14.17.3 | additional information | acetyl-L-Phe-Gly, acetyl-L-Phe-L-Phe-Gly, or (S)-O-acetyl-mandelyl-Gly, and stereoisomers are alpha-hydroxylated but not alpha-amidated | Bos taurus | ? | - |
? | |
1.14.17.3 | peptidylglycine + ascorbate + O2 | - |
Bos taurus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
1.14.17.3 | peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Bos taurus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
4.3.2.5 | (R)-2-phenylbutyl-alpha-hydroxy-Gly | - |
Bos taurus | (R)-2-phenylbutyl-NH2 + glyoxylate | - |
? | |
4.3.2.5 | (R)-O-acetyl-mandelyl-alpha-hydroxyglycine | - |
Bos taurus | (R)-O-acetyl-mandelyl-NH2 + glyoxylate | - |
? | |
4.3.2.5 | (S)-2-phenylbutyl-alpha-hydroxy-Gly | - |
Bos taurus | (S)-2-phenylbutyl-NH2 + glyoxylate | - |
? | |
4.3.2.5 | (S)-O-acetyl-mandelyl-alpha-hydroxyglycine | - |
Bos taurus | (S)-O-acetyl-mandelyl-NH2 + glyoxylate | - |
? | |
4.3.2.5 | acetyl-D-Phe-alpha-hydroxyglycine | - |
Bos taurus | acetyl-D-Phe-NH2 + glyoxylate | - |
? | |
4.3.2.5 | acetyl-L-Phe-alpha-hydroxyglycine | - |
Bos taurus | acetyl-L-Phe-NH2 + glyoxylate | - |
? | |
4.3.2.5 | N-benzoyl-alpha-hydroxy-Gly | - |
Bos taurus | benzamide + glyoxylate | - |
? | |
4.3.2.5 | trinitrophenyl-D-Tyr-L-Val-alpha-hydroxy-Gly | - |
Bos taurus | trinitrophenyl-D-Tyr-L-Val-NH2 + glyoxylate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.17.3 | More | EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PHM), 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PHL) | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.17.3 | ascorbate | - |
Bos taurus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.17.3 | 0.002 | - |
acetyl-L-Phe-Gly | - |
Bos taurus | |
1.14.17.3 | 0.045 | - |
acetyl-L-Phe-OCH2COOH | - |
Bos taurus | |
1.14.17.3 | 0.0598 | - |
acetyl-L-Leu-OCH2COOH | - |
Bos taurus | |
1.14.17.3 | 0.522 | - |
acetyl-DL-Phe-OCH2COOH | - |
Bos taurus | |
1.14.17.3 | 0.58 | - |
acetyl-L-Phe-D-Phe-Gly | - |
Bos taurus | |
1.14.17.3 | 1.25 | - |
acetyl-Gly-OCH2COOH | - |
Bos taurus | |
1.14.17.3 | 1.3 | - |
acetyl-D-Phe-Gly | - |
Bos taurus | |
1.14.17.3 | 2.11 | - |
acetyl-D-Leu-OCH2COOH | - |
Bos taurus | |
1.14.17.3 | 2.25 | - |
acetyl-D-Phe-OCH2COOH | - |
Bos taurus | |
4.3.2.5 | 0.28 | - |
(S)-2-phenylbutyl-alpha-hydroxy-Gly | 37°C, pH 6.5 | Bos taurus | |
4.3.2.5 | 0.31 | - |
(R)-2-phenylbutyl-alpha-hydroxy-Gly | 37°C, pH 6.5 | Bos taurus | |
4.3.2.5 | 0.77 | - |
phenylacetyl-alpha-hydroxy-Gly | 37°C, pH 6.5 | Bos taurus |