Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bell, J.; Ash, D.E.; Snyder, L.M.; Kulathila, R.; Blackburn, N.J.; Merkler, D.J.
    Structural and functional investigations on the role of zinc in bifunctional rat peptidylglycine alpha-amidating enzyme (1997), Biochemistry, 36, 16239-16246.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.3 medullary thyroid alpha-AE, type A, expression in chinese hamster ovary cells Rattus norvegicus

General Stability

EC Number General Stability Organism
1.14.17.3 Cu2+ can restore EDTA-treated only the alpha-hydroxylating activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.3 EDTA recombinant enzyme, alpha-hydroxylation activity can be restored by Mn2+, Zn2+, Cd2+, and Co2+, but not by Ca2+, Cu2+, Mg2+, and Fe3+ Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.17.3 extracellular recombinant enzyme secreted into medium Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+ required for alpha-hydroxylation step Rattus norvegicus
1.14.17.3 Zn2+ required for alpha-amidation step Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Rattus norvegicus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Rattus norvegicus P14925 bifunctional alpha-AE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.3 type A alpha-AE, recombinant from chinese hamster ovary cells Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 medullary thyroid carcinoma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 dansyl-D-Tyr-Val-Gly + ascorbate + O2
-
 Rattus norvegicus dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 N-dansyl-Tyr-Val-Gly + ascorbate + O2
-
 Rattus norvegicus (S)-N-dansyl-Tyr-Val-alpha-hydroxyglycine + dehydroascorbate + H2O
-
 ir
1.14.17.3 peptidylglycine + ascorbate + O2
-
 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.17.3 alpha-AE peptidylglycine alpha-amidating enzyme Rattus norvegicus
1.14.17.3 More the 2-step process is catalyzed by only 1 enzyme called type A peptidylglycine alpha-amidating enzyme Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate dependent on Rattus norvegicus