EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.17.3 | dexamethasone | increases gene expression 1.6fold, northern blot analysis | Rattus norvegicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.17.3 | myoblast enzyme, expression in H9c2 cells | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.17.3 | Cu2+ | - |
Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.17.3 | additional information | - |
PAM in particulate fraction of H9c2 cells contains 86, 76, and 46 kDa proteins, soluble PAM fraction contains 110, 86, and 46 kDa proteins | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.3 | peptidylglycine + ascorbate + O2 | Rattus norvegicus | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.17.3 | Rattus norvegicus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.17.3 | heart | myoblast, H9c2 cells | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.17.3 | additional information | - |
very low activity in H9c2 cells, no ascorbate in assay mixture | Rattus norvegicus |
1.14.17.3 | 0.0000000004 | - |
H9c2 cells | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.3 | alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
1.14.17.3 | monoiodo-alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | monoiodo-alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
1.14.17.3 | additional information | EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PMH) and 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase | Rattus norvegicus | ? | - |
? | |
1.14.17.3 | peptidylglycine + ascorbate + O2 | - |
Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
1.14.17.3 | peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.17.3 | More | EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PHM), 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PHL) | Rattus norvegicus |