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Literature summary extracted from

  • Girard, B.; Ouafik, L.; Boudouresque, F.
    Characterization and regulation of peptidylglycine alpha-amidating monooxygenase (PAM) expression in H9c2 cardiac myoblasts (1999), Cell Tissue Res., 298, 489-497.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.17.3 dexamethasone increases gene expression 1.6fold, northern blot analysis Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.3 myoblast enzyme, expression in H9c2 cells Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+
-
Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.17.3 additional information
-
PAM in particulate fraction of H9c2 cells contains 86, 76, and 46 kDa proteins, soluble PAM fraction contains 110, 86, and 46 kDa proteins Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Rattus norvegicus
-
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 heart myoblast, H9c2 cells Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.3 additional information
-
very low activity in H9c2 cells, no ascorbate in assay mixture Rattus norvegicus
1.14.17.3 0.0000000004
-
H9c2 cells Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2
-
Rattus norvegicus alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
?
1.14.17.3 monoiodo-alpha-N-acetyl-Tyr-Val-Gly + ascorbate + O2
-
Rattus norvegicus monoiodo-alpha-N-acetyl-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
?
1.14.17.3 additional information EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PMH) and 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase Rattus norvegicus ?
-
?
1.14.17.3 peptidylglycine + ascorbate + O2
-
Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.17.3 More EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PHM), 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PHL) Rattus norvegicus