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Literature summary extracted from

  • Boudreault, A.; Castellucci, V.F.; Chretien, M.; Lazure, C.
    Identification, purification, and characterization of the molecular forms of Aplysia californica peptidylglycine alpha-amidating enzyme (1996), J. Neurochem., 66, 2596-2605.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.3 Ni2+
-
 Aplysia californica
1.14.17.3 Zn2+
-
 Aplysia californica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.17.3 cytoplasm soluble form Aplysia californica 5737
-
1.14.17.3 membrane particulate form, except for abdominal ganglia Aplysia californica 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+ 2fold increase in activity Aplysia californica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.17.3 50000
-
 1 * 50000, membrane-bound form, SDS-PAGE Aplysia californica
1.14.17.3 60000
-
 gel filtration Aplysia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Aplysia californica
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Aplysia californica
-
 marine mollusc
-
1.14.17.3 Aplysia californica
-
 2 forms: a soluble and a membrane-bound
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.3 membrane-bound form from atrial gland Aplysia californica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 atrial gland
-
 Aplysia californica
-
1.14.17.3 ganglion abdominal and pleuropedal Aplysia californica
-
1.14.17.3 heart
-
 Aplysia californica
-
1.14.17.3 additional information tissue distribution, overview Aplysia californica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.3 additional information
-
-
 Aplysia californica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 D-Tyr-Val-Gly + ascorbate + O2 synthetic peptid Aplysia californica D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2
-
 Aplysia californica peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 requirement for O2 Aplysia californica peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Aplysia californica peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.17.3 monomer 1 * 50000, membrane-bound form, SDS-PAGE Aplysia californica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.3 37
-
 assay at Aplysia californica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.3 6 7.5
-
 Aplysia californica

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate dependent on Aplysia californica