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Literature summary extracted from

  • Katapodis, A.G.; May, S.W.
    A new facile trinitrophenylated substrate for peptide alpha-amidation and its use to characterize PAM activity in chromaffin granules (1988), Biochem. Biophys. Res. Commun., 151, 499-505.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.17.3 secretory granule chromaffin granules Bos taurus 30141
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Bos taurus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 pituitary gland neurointermediate Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.3 0.0033
-
 alpha-amidating lyase activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 dansyl-D-Tyr-Val-Gly + ascorbate + O2
-
 Bos taurus dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2
-
 Bos taurus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Bos taurus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 trinitrophenyl-D-Tyr-Val-Gly + ascorbate + O2 COOH-terminal glycine Bos taurus trinitrophenyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate dependent on Bos taurus