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Literature summary extracted from

  • Whittle, E.; Shanklin, J.
    Engineering D9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation mutagenesis and logical redesign of the castor DELTA9-18:0-ACP desaturase (2001), J. Biol. Chem., 276, 21500-21505.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.2 T117R/G188L 82fold higher specificity for palmitoyl-[acyl-carrier protein] with respect to wild-type Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.2 0.00046
-
 octadecanoyl-[acyl-carrier protein] wild type enzyme, pH and temperature not specified in the publication Ricinus communis
1.14.19.2 0.00055
-
 hexadecanoyl-[acyl-carrier protein] T117R/G188L mutant enzyme Ricinus communis
1.14.19.2 0.00055
-
 hexadecanoyl-[acyl-carrier protein] mutant enzyme T117R/G188L, pH and temperature not specified in the publication Ricinus communis
1.14.19.2 0.00098
-
 octadecanoyl-[acyl-carrier protein] T117R/G188L mutant enzyme Ricinus communis
1.14.19.2 0.00098
-
 octadecanoyl-[acyl-carrier protein] mutant enzyme T117R/G188L, pH and temperature not specified in the publication Ricinus communis
1.14.19.2 0.001
-
 tetradecanoyl-[acyl-carrier protein] T117R/G188L mutant enzyme Ricinus communis
1.14.19.2 0.00464
-
 tetradecanoyl-[acyl-carrier protein]
-
 Ricinus communis
1.14.19.2 0.005
-
 hexadecanoyl-[acyl-carrier protein] wild type enzyme, pH and temperature not specified in the publication Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.2 Ricinus communis P22337
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.2 hexadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2 + H+
-
 Ricinus communis 9-hexadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
-
 r
1.14.19.2 octadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2 + H+
-
 Ricinus communis 9-octadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
-
 r
1.14.19.2 tetradecanoyl-[acyl-carrier protein] + reduced acceptor + O2
-
 Ricinus communis 9-tetradecenoyl-[acyl-carrier protein] + acceptor + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.19.2 0.0065
-
 tetradecanoyl-[acyl-carrier protein]
-
 Ricinus communis
1.14.19.2 0.0273
-
 tetradecanoyl-[acyl-carrier protein] T117R/G188L mutant enzyme Ricinus communis
1.14.19.2 0.0467
-
 hexadecanoyl-[acyl-carrier protein]
-
 Ricinus communis
1.14.19.2 0.0817
-
 octadecanoyl-[acyl-carrier protein] T117R/G188L mutant enzyme Ricinus communis
1.14.19.2 0.422
-
 hexadecanoyl-[acyl-carrier protein] T117R/G188L mutant enzyme Ricinus communis
1.14.19.2 0.705
-
 octadecanoyl-[acyl-carrier protein]
-
 Ricinus communis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.2 Ferredoxin
-
 Ricinus communis