EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.3 | expression of wild-type, A181T/A188G/Y189F/S205N/L206T/G207A, A188G/Y189F, A181T/A200F and A181T/A200F/S205N/L206T/G207A mutant enzyme in Escherichia coli | Thunbergia alata |
1.14.19.26 | expression of wild-type and mutant enzymes in Escherichia coli | Thunbergia alata |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.19.2 | L118F/P179I | 15fold higher activity with palmitoyl-[acyl-carrier protein] than wild-type enzyme, low DELTA10 desaturase activity | Ricinus communis |
1.14.19.3 | A181T/A188G/Y189F/S205N/L206T/G207A | compared to wild-type: reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, strong DELTA9 desaturase activity with stearoyl-[acyl-carrier protein] as substrate, exhibits DELTA9 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate | Thunbergia alata |
1.14.19.3 | A181T/A200F | increase in DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, strong DELTA9 desaturase activity with stearoyl-[acyl-carrier protein] as substrate | Thunbergia alata |
1.14.19.3 | A181T/A200F/S205N/L206T/G207A | reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, very low DELTA9 desaturase activity, no DELTA6 desaturase activity with stearoyl-[acyl-carrier protein] as substrate | Thunbergia alata |
1.14.19.3 | A188G/Y189F | reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate | Thunbergia alata |
1.14.19.26 | A181T/A188G/Y189F/S205N/L206T/G207A | the mutation yields an enzyme that functions primarily as a DELTA9-18:0-[acyl-carrier protein] desaturase. This enzyme displays only DELTA9 desaturase activity with 18:0-[acyl-carrier protein] and is nearly 4-fold more active with this substrate than with 16:0-[acyl-carrier protein]. Like mutant A181TyA200F, this enzyme retains DELTA6 desaturase activity with 16:0-[acyl-carrier protein] | Thunbergia alata |
1.14.19.26 | A181T/A200F | the mutation gives rise to an enzyme that catalyzed primarily the DELTA9 desaturation of 18:0-[acyl-carrier protein], but functions as a DELTA6 desaturase with 16:0-[acyl-carrier protein] | Thunbergia alata |
1.14.19.26 | A181T/A200F/S205N/L206T/G207A | replacement of specific amino acid residues in a DELTA6-palmitoyl (16:0)-[acyl-carrier protein] desaturase with their equivalents from a DELTA9-stearoyl (18:0)-[acyl-carrier protein] desaturase. The A181T/A200F/S205N/L206T/G207A mutant enzyme is functions principally as a DELTA9-18:0-[acyl-carrier protein] desaturase | Thunbergia alata |
1.14.19.26 | A188G/Y189F | mutant with broadened fatty acid chain-length specificity | Thunbergia alata |
1.14.19.26 | additional information | replacement of specific amino acid residues in a DELTA6-palmitoyl (16:0)-[acyl-carrier protein] desaturase with their equivalents from a DELTA9-stearoyl (18:0)-[acyl-carrier protein] desaturase, mutant enzymes are identified that have altered fatty acid chain-length specificities or that can insert double bonds into either the DELTA6 or DELTA9 positions of 16:0- and 18:0-[acyl-carrier protein] | Thunbergia alata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.26 | additional information | - |
palmitoyl-[acyl-carrier protein] | Km-values determined for the wild-type enzyme and mutant A188G/Y189F are in the range of 0.2 to 0.6 mM with both 16:0- and 18:0-[acyl-carrier protein], suggesting that, at least in the case of these enzymes, changes in the substrate binding properties can be discounted as an underlying cause of differences in activities | Thunbergia alata |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.26 | palmitoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Thunbergia alata | - |
(Z)-hexadec-6-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.2 | Ricinus communis | P22337 | - |
- |
1.14.19.3 | Thunbergia alata | - |
black-eyed Susan vine | - |
1.14.19.26 | Thunbergia alata | Q41510 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.19.3 | recombinant enzyme | Thunbergia alata |
1.14.19.26 | - |
Thunbergia alata |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.19.3 | 0.1 | - |
substrate palmitoyl-[acyl-carrier protein] | Thunbergia alata |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.3 | palmitoyl-[acyl-carrier protein] + reduced acceptor + O2 | - |
Thunbergia alata | 6-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
1.14.19.3 | stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | - |
Thunbergia alata | 6-octadecenoyl-[acyl-carrier protein] + 9-octadecenoyl-[acyl-carrier protein]acceptor + H2O | ratio of activity: 2/1 | ? | |
1.14.19.26 | palmitoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Thunbergia alata | (Z)-hexadec-6-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.26 | DELTA6-16:0-ACP desaturase | - |
Thunbergia alata |
1.14.19.26 | DELTA6-palmitoyl (16:0)-ACP desaturase | - |
Thunbergia alata |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.2 | Ferredoxin | - |
Ricinus communis | |
1.14.19.3 | Ferredoxin | - |
Thunbergia alata |