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Literature summary extracted from
- Purification and characterization of the stearoyl-acyl carrier protein desaturase and the acyl-acyl carrier protein thioesterase from maturing seeds of safflower (1982), J. Biol. Chem., 257, 12141-12147.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.2 | catalase | 3fold stimulation of purified enzyme, 4-5fold increase of activity in crude extracts | Carthamus tinctorius |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.2 | 0.00038 | - |
stearoyl-[acyl-carrier protein] | - |
Carthamus tinctorius |  |
| 1.14.19.2 | 0.00051 | - |
palmitoyl-[acyl-carrier protein] | - |
Carthamus tinctorius | |
| 1.14.19.2 | 0.0083 | - |
stearoyl-CoA | - |
Carthamus tinctorius | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.2 | 36000 | - |
2 * 36000, SDS-PAGE | Carthamus tinctorius |
| 1.14.19.2 | 68000 | - |
gel filtration | Carthamus tinctorius |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + electron donor + O2 | Carthamus tinctorius | involved in oleic acid biosynthesis | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.2 | Carthamus tinctorius | P22243 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.19.2 | - |
Carthamus tinctorius |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.19.2 | seed | - |
Carthamus tinctorius | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.2 | 0.11 | - |
- |
Carthamus tinctorius |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.19.2 | -70°C, 50% glycerol, 0.1% bovine serum albumin, 100 mM potassium phosphate buffer, pH 6.8, at least 2 months, no loss of activity | Carthamus tinctorius |
| 1.14.19.2 | 4°C, overnight, 30% loss of activity | Carthamus tinctorius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.2 | palmitoyl-[acyl-carrier protein] + reduced acceptor + O2 | 1% of activity with stearoyl-[acyl-carrier-protein] | Carthamus tinctorius | 9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-CoA + electron donor + O2 | 5% of activity with stearoyl-[acyl-carrier-protein] | Carthamus tinctorius | 9-octadecenoyl-CoA + acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + electron donor + O2 | involved in oleic acid biosynthesis | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | enzyme is specific for stearoyl-CoA | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.19.2 | dimer | 2 * 36000, SDS-PAGE | Carthamus tinctorius |
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