BRENDA - Enzyme Database show

The same rat DELTA6-desaturase not only acts on 18- but also on 24-carbon fatty acids in very-long-chain polyunsaturated fatty acid biosynthesis

D'Andrea, S.; Guillou, H.; Jan, S.; Catheline, D.; Thibault, J.N.; Bouriel, M.; Rioux, V.; Legrand, P.; Biochem. J. 364, 49-55 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.3
DELTA6-desaturase gene identified in human chromosome 11
Homo sapiens
1.14.19.3
plasmid coding for rat delta6-desaturase constructed using pCMV for expression in mammalian cells, rat delta6-desaturase sequence, GenBank accession number AB02 1980 PCR amplified, expressed by transiently transforming COS-7 cells
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.3
microsome
-
Homo sapiens
-
-
1.14.19.3
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.3
45000
-
recombinant DELTA6-desaturase expressed in COS-7 cells, analysed by Western blot
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.3
linoleic acid + AH2 + O2
Homo sapiens
-
gamma-linolenic acid + A + H2O
-
-
?
1.14.19.3
linoleic acid + AH2 + O2
Rattus norvegicus
-
gamma-linolenic acid + A + H2O
-
-
?
1.14.19.3
linoleic acid + AH2 + O2
Rattus norvegicus Sprague-Dawley
-
gamma-linolenic acid + A + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.3
Homo sapiens
-
human
-
1.14.19.3
Rattus norvegicus
-
-
-
1.14.19.3
Rattus norvegicus Sprague-Dawley
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.3
brain
-
Homo sapiens
-
1.14.19.3
brain
-
Rattus norvegicus
-
1.14.19.3
liver
-
Homo sapiens
-
1.14.19.3
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.3
24-carbon fatty acid + AH2 + O2
-
438422
Rattus norvegicus
? + A + H2O
-
-
-
?
1.14.19.3
24-carbon fatty acid + AH2 + O2
-
438422
Rattus norvegicus Sprague-Dawley
? + A + H2O
-
-
-
?
1.14.19.3
alpha-linolenic acid + AH2 + O2
-
438422
Homo sapiens
stearidonic acid + A + H2O
-
438422
Homo sapiens
?
1.14.19.3
alpha-linolenic acid + AH2 + O2
-
438422
Rattus norvegicus
stearidonic acid + A + H2O
-
438422
Rattus norvegicus
?
1.14.19.3
alpha-linolenic acid + AH2 + O2
-
438422
Rattus norvegicus Sprague-Dawley
stearidonic acid + A + H2O
-
438422
Rattus norvegicus Sprague-Dawley
?
1.14.19.3
linoleic acid + AH2 + O2
-
438422
Homo sapiens
gamma-linolenic acid + A + H2O
-
-
-
?
1.14.19.3
linoleic acid + AH2 + O2
-
438422
Rattus norvegicus
gamma-linolenic acid + A + H2O
-
-
-
?
1.14.19.3
linoleic acid + AH2 + O2
-
438422
Rattus norvegicus Sprague-Dawley
gamma-linolenic acid + A + H2O
-
-
-
?
1.14.19.3
octadeca-9,12-dienoic acid + AH2 + O2
-
438422
Homo sapiens
gamma-linolenic acid + A + H2O
-
438422
Homo sapiens
?
1.14.19.3
octadeca-9,12-dienoic acid + AH2 + O2
-
438422
Rattus norvegicus
gamma-linolenic acid + A + H2O
-
438422
Rattus norvegicus
?
1.14.19.3
octadeca-9,12-dienoic acid + AH2 + O2
-
438422
Rattus norvegicus Sprague-Dawley
gamma-linolenic acid + A + H2O
-
438422
Rattus norvegicus Sprague-Dawley
?
1.14.19.3
octadecanoate + AH2 + O2
-
438422
Rattus norvegicus
? + A + H2O
-
-
-
?
1.14.19.3
octadecanoate + AH2 + O2
-
438422
Rattus norvegicus Sprague-Dawley
? + A + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.3
DELTA6-desaturase gene identified in human chromosome 11
Homo sapiens
1.14.19.3
plasmid coding for rat delta6-desaturase constructed using pCMV for expression in mammalian cells, rat delta6-desaturase sequence, GenBank accession number AB02 1980 PCR amplified, expressed by transiently transforming COS-7 cells
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.3
microsome
-
Homo sapiens
-
-
1.14.19.3
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.3
45000
-
recombinant DELTA6-desaturase expressed in COS-7 cells, analysed by Western blot
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.3
linoleic acid + AH2 + O2
Homo sapiens
-
gamma-linolenic acid + A + H2O
-
-
?
1.14.19.3
linoleic acid + AH2 + O2
Rattus norvegicus
-
gamma-linolenic acid + A + H2O
-
-
?
1.14.19.3
linoleic acid + AH2 + O2
Rattus norvegicus Sprague-Dawley
-
gamma-linolenic acid + A + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.3
brain
-
Homo sapiens
-
1.14.19.3
brain
-
Rattus norvegicus
-
1.14.19.3
liver
-
Homo sapiens
-
1.14.19.3
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.3
24-carbon fatty acid + AH2 + O2
-
438422
Rattus norvegicus
? + A + H2O
-
-
-
?
1.14.19.3
24-carbon fatty acid + AH2 + O2
-
438422
Rattus norvegicus Sprague-Dawley
? + A + H2O
-
-
-
?
1.14.19.3
alpha-linolenic acid + AH2 + O2
-
438422
Homo sapiens
stearidonic acid + A + H2O
-
438422
Homo sapiens
?
1.14.19.3
alpha-linolenic acid + AH2 + O2
-
438422
Rattus norvegicus
stearidonic acid + A + H2O
-
438422
Rattus norvegicus
?
1.14.19.3
alpha-linolenic acid + AH2 + O2
-
438422
Rattus norvegicus Sprague-Dawley
stearidonic acid + A + H2O
-
438422
Rattus norvegicus Sprague-Dawley
?
1.14.19.3
linoleic acid + AH2 + O2
-
438422
Homo sapiens
gamma-linolenic acid + A + H2O
-
-
-
?
1.14.19.3
linoleic acid + AH2 + O2
-
438422
Rattus norvegicus
gamma-linolenic acid + A + H2O
-
-
-
?
1.14.19.3
linoleic acid + AH2 + O2
-
438422
Rattus norvegicus Sprague-Dawley
gamma-linolenic acid + A + H2O
-
-
-
?
1.14.19.3
octadeca-9,12-dienoic acid + AH2 + O2
-
438422
Homo sapiens
gamma-linolenic acid + A + H2O
-
438422
Homo sapiens
?
1.14.19.3
octadeca-9,12-dienoic acid + AH2 + O2
-
438422
Rattus norvegicus
gamma-linolenic acid + A + H2O
-
438422
Rattus norvegicus
?
1.14.19.3
octadeca-9,12-dienoic acid + AH2 + O2
-
438422
Rattus norvegicus Sprague-Dawley
gamma-linolenic acid + A + H2O
-
438422
Rattus norvegicus Sprague-Dawley
?
1.14.19.3
octadecanoate + AH2 + O2
-
438422
Rattus norvegicus
? + A + H2O
-
-
-
?
1.14.19.3
octadecanoate + AH2 + O2
-
438422
Rattus norvegicus Sprague-Dawley
? + A + H2O
-
-
-
?