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Literature summary extracted from

  • Bernhardt, F.H.; Heymann, E.; Traylor, P.S.
    Chemical and spectral properties of putidamonooxin, the iron-containing and acid-labile-sulfur-containing monooxygenase of a 4-methoxybenzoate O-demethylase from Pseudomonas putida (1978), Eur. J. Biochem., 92, 209-223.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.99.15 Putidamonooxin
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.15 0.00007
-
 4-Methoxybenzoate
-
 Pseudomonas putida
1.14.99.15 0.0003
-
 3-Nitro-4-methoxybenzoate
-
 Pseudomonas putida
1.14.99.15 0.0058
-
 4-methylaminobenzoate
-
 Pseudomonas putida
1.14.99.15 0.009
-
 4-Methylbenzoate
-
 Pseudomonas putida
1.14.99.15 0.024
-
 4-trifluoromethylbenzoate
-
 Pseudomonas putida
1.14.99.15 0.029
-
 4-hydroxybenzoate
-
 Pseudomonas putida
1.14.99.15 0.055
-
 O2 + benzoate Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.15 Fe2+ both enzyme components are iron-sulfur proteins Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.15 41500
-
 3-4 * 41500, putidamonooxin, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.15 4-methoxybenzoate + NADH + O2 Pseudomonas putida
-
 4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.15 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.15
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.15 3-nitro-4-methoxybenzoate + NADH + O2
-
 Pseudomonas putida 3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
1.14.99.15 4-hydroxybenzoate + NADH + O2
-
 Pseudomonas putida 3,4-dihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.99.15 4-methoxybenzoate + NADH + O2
-
 Pseudomonas putida 4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
1.14.99.15 4-methylbenzoate + NADH + O2 p-toluate, partial uncoupler Pseudomonas putida 4-carboxybenzylalcohol + NAD+ + H2O in the uncoupled part of the reaction, p-toluate is not hydroxylated and H2O2 is a product, too ?
1.14.99.15 4-trifluoromethylbenzoate + NADH + O2
-
 Pseudomonas putida ?
-
 ?
1.14.99.15 additional information overview: substrates that are not oxygenized while NADH-oxidation and O2-consumption are catalyzed, such as: benzoate, 3-chlorobenzoate, 4-chlorobenzoate, 2-hydroxybenzoate, 4-bromobenzoate, 2-aminobenzoate, 3-aminobenzoate, 4-trifluoromethylbenzoate, 4-tert-butylbenzoate Pseudomonas putida additional information uncoupling substrates are not oxygenized, NAD+ and H2O2 being the only products of the reaction ?
1.14.99.15 N-methyl-4-aminobenzoate + NADH + O2
-
 Pseudomonas putida 4-aminobenzoate + NAD+ + H2O + formaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.15 oligomer 3-4 * 41500, putidamonooxin, SDS-PAGE Pseudomonas putida
1.14.99.15 oligomer 3-4 * 33000-45000, putidamonooxin, SDS-PAGE Pseudomonas putida