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Literature summary extracted from
- Kinetic studies on a 4-methoxybenzoate O-demethylase from Pseudomonas putida (1977), Eur. J. Biochem., 72, 107-115.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.15 | Putidamonooxin | - |
Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.15 | 4-(2-Pyridylazo)resorcinol | - |
Pseudomonas putida |  |
| 1.14.99.15 | 4-tert-Butylbenzoate | competitive inhibitor of the O-demethylation of 3-nitro-4-methoxybenzoate, hinders O2-binding or O2-activation | Pseudomonas putida | |
| 1.14.99.15 | 8-hydroxyquinoline | - |
Pseudomonas putida | |
| 1.14.99.15 | Amytal | - |
Pseudomonas putida | |
| 1.14.99.15 | Atebrin | - |
Pseudomonas putida | |
| 1.14.99.15 | Bathocuproinedisulfonate | - |
Pseudomonas putida | |
| 1.14.99.15 | bathophenanthrolinedisulfate | preferentially inhibiting putidamonooxin | Pseudomonas putida | |
| 1.14.99.15 | Cd2+ | - |
Pseudomonas putida | |
| 1.14.99.15 | Co2+ | - |
Pseudomonas putida | |
| 1.14.99.15 | Cu2+ | - |
Pseudomonas putida | |
| 1.14.99.15 | Cumylhydroperoxide | - |
Pseudomonas putida | |
| 1.14.99.15 | Hg2+ | - |
Pseudomonas putida | |
| 1.14.99.15 | Iodosobenzene | - |
Pseudomonas putida | |
| 1.14.99.15 | Ni2+ | - |
Pseudomonas putida | |
| 1.14.99.15 | p-chloromercuribenzoate | - |
Pseudomonas putida | |
| 1.14.99.15 | rotenone | - |
Pseudomonas putida | |
| 1.14.99.15 | Zn2+ | - |
Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.99.15 | 0.00007 | - |
4-Methoxybenzoate | - |
Pseudomonas putida | |
| 1.14.99.15 | 0.0003 | - |
3-Nitro-4-methoxybenzoate | - |
Pseudomonas putida | |
| 1.14.99.15 | 0.00063 | - |
NADH | reconstituted enzyme | Pseudomonas putida | |
| 1.14.99.15 | 0.0058 | - |
4-methylaminobenzoate | - |
Pseudomonas putida | |
| 1.14.99.15 | 0.009 | - |
4-Methylbenzoate | - |
Pseudomonas putida | |
| 1.14.99.15 | 0.024 | - |
4-trifluoromethylbenzoate | - |
Pseudomonas putida | |
| 1.14.99.15 | 0.029 | - |
4-hydroxybenzoate | - |
Pseudomonas putida | |
| 1.14.99.15 | 0.055 | - |
O2 | + benzoate | Pseudomonas putida | |
| 1.14.99.15 | 0.14 | - |
NADPH | reconstituted enzyme | Pseudomonas putida | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.15 | Fe2+ | both enzyme components are iron-sulfur proteins | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.15 | 4-methoxybenzoate + NADH + O2 | Pseudomonas putida | - |
4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.15 | Pseudomonas putida | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.15 | 0.018 | - |
superoxide anion instead of substrate, putidamonooxin | Pseudomonas putida |
| 1.14.99.15 | 0.192 | - |
3-nitro-4-methoxybenzoate, putidamonooxin | Pseudomonas putida |
| 1.14.99.15 | 0.27 | - |
4-methoxybenzoate, putidamonooxin | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.15 | 3-nitro-4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
| 1.14.99.15 | 3-nitro-4-methoxybenzoate + NADPH + O2 | - |
Pseudomonas putida | 3-nitro-4-hydroxybenzoate + NADP+ + H2O + formaldehyde | - |
r | |
| 1.14.99.15 | 4-hydroxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxybenzoate + NAD+ + H2O | ring hydroxylation | ? | |
| 1.14.99.15 | 4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
| 1.14.99.15 | 4-methylbenzoate + NADH + O2 | - |
Pseudomonas putida | 4-carboxybenzylalcohol + NAD+ + H2O | - |
? | |
| 1.14.99.15 | 4-trifluoromethylbenzoate + NADH + O2 | - |
Pseudomonas putida | ? | - |
? | |
| 1.14.99.15 | N-methyl-4-aminobenzoate + NADH + O2 | - |
Pseudomonas putida | 4-aminobenzoate + NAD+ + H2O + formaldehyde | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.15 | 30 | - |
assay at | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.15 | FMN | - |
Pseudomonas putida | |
| 1.14.99.15 | NADH | requirement, can be replaced by NADPH with 40% efficiency | Pseudomonas putida | |
| 1.14.99.15 | NADPH | can replace NADH with 40% efficiency | Pseudomonas putida | |
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