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Literature summary extracted from

  • Sugiura, I.; Furie, B.; Walsh, C.T.; Furie, B.C.
    Propeptide and glutamate-containing substrates bound to the vitamin K-dependent carboxylase convert its vitamin K epoxidase function from an inactive to an active state (1997), Proc. Natl. Acad. Sci. USA, 94, 9069-9074.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.99.20 glutamate-containing substrates bound to the vitamin K-dependent carboxylase converts its vitamin K epoxidase function from an inactive to an active state Bos taurus
1.14.99.20 propeptide bound to the vitamin K-dependent carboxylase converts its vitamin K epoxidase function from an inactive to an active state Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.20 expressed in chinese hamster ovary cells CHO-Dukx-B11 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.20 microsome microsomal recombinant protein preparation from chinese hamster ovary cells Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.20 94000
-
 x * 94000, SDS-PAGE, wild type enzyme Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.20 phylloquinone + electron donor + O2 Bos taurus
-
 2,3-epoxyphylloquinone + oxidized electron + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.20 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.20
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.99.20 phylloquinone + reduced acceptor + O2 = 2,3-epoxyphylloquinone + acceptor + H2O propeptide and glutamate-containing substrates bound to the vitamin K-dependent carboxylase convert its vitamin K epoxidase function from an inactive to an active state Bos taurus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.20 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.14.99.20 -80°C, 20% glycerol Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.20 phylloquinone + electron donor + O2
-
 Bos taurus 2,3-epoxyphylloquinone + oxidized electron donor + H2O
-
 ?
1.14.99.20 phylloquinone + electron donor + O2
-
 Bos taurus 2,3-epoxyphylloquinone + oxidized electron + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.20 ? x * 94000, SDS-PAGE, wild type enzyme Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.20 25
-
 enzyme assay Bos taurus