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Literature summary extracted from

  • Hubbard, B.R.; Ulrich, M.M.W.; Jacobs, M.; Vermeer, C.; Walsh, C.; Furie, B.; Furie, B.C.
    Vitamin K-dependent carboxylase: affinity purification from bovine liver by using a synthetic propeptide containing the gamma-carboxylation recognition site (1989), Proc. Natl. Acad. Sci. USA, 86, 6893-6897.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.90 anti-carboxylase antiserum effect of anti-carboxylase antiserum on carboxylase activity: under the conditions employed the carboxylation is inhibited by 80%, with parallel inhibition of CO2 incorporation into FLEEL and proPT28 (synthetic peptide) Bos taurus
4.1.1.90 additional information in the presence of high concentrations of propeptide, only minimal carboxylase activity is measurable. Antibodies to the protein inhibit the carboxylase activity in crude preparations Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.20 microsome
-
 Bos taurus
-
-
4.1.1.90 membrane
-
 Bos taurus 16020
-
4.1.1.90 microsome
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.90 additional information calcium-binding protein Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.20 77000
-
 x * 77000, SDS-PAGE Bos taurus
4.1.1.90 77000
-
 single major band on SDS gel electrophoresis. The eluted protein contains both stable vitamin K-dependent carboxylase and vitamin K epoxidase activity Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.90 L-glutamate + CO2 + O2 + vitamin K hydroquinone Bos taurus
-
 gamma-carboxy L-glutamate + vitamin K epoxide + H2O
-
 ?
4.1.1.90 additional information Bos taurus enzyme catalyzes the posttranslational modification of specific glutamic acid residues to form gamma-carboxygutamic acid residues within the vitamin K-dependent proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.20 Bos taurus
-
-
-
4.1.1.90 Bos taurus Q07175
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.20 partially Bos taurus
4.1.1.90 affinity chromatography Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.99.20 phylloquinone + reduced acceptor + O2 = 2,3-epoxyphylloquinone + acceptor + H2O vitamin K-dependent carboxylase activity and vitamin K epoxidase activity are properties of the same protein Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.20 liver
-
 Bos taurus
-
4.1.1.90 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.20 0.00466
-
-
 Bos taurus
4.1.1.90 additional information
-
 specific activities of the partially purified microsomes and the homogeneous vitamin K-dependent carboxylase are 2.77 and 279.6 nmol of vitamin K epoxide per h per mg of protein, a 101fold purification of the vitamin K epoxidase activity from partially purified microsomes Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.20 Phe-Leu-Glu-Glu-Leu + reduced acceptor + O2
-
 Bos taurus ?
-
 ?
4.1.1.90 FLEEL + CO2 + O2 + vitamin K hydroquinone
-
 Bos taurus ? + vitamin K epoxide + H2O
-
 ?
4.1.1.90 L-glutamate + CO2 + O2 + vitamin K hydroquinone
-
 Bos taurus gamma-carboxy L-glutamate + vitamin K epoxide + H2O
-
 ?
4.1.1.90 additional information enzyme catalyzes the posttranslational modification of specific glutamic acid residues to form gamma-carboxygutamic acid residues within the vitamin K-dependent proteins Bos taurus ?
-
 ?
4.1.1.90 proPT28 + CO2 + O2 + vitamin K hydroquinone proPT28: synthetic peptide is designated by the following nomenclature: pro indicates the presence of the propeptide sequence, PT indicates prothrombin, the protein on which the peptide sequence is based, and 28 indicates the number of amino acid residues in the peptide Bos taurus ? + vitamin K epoxide + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.20 ? x * 77000, SDS-PAGE Bos taurus
4.1.1.90 monomer 1 * 77000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.90 glutamate carboxylase
-
 Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.99.20 0.00667
-
 Phe-Leu-Glu-Glu-Leu
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.90 vitamin K
-
 Bos taurus