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Literature summary extracted from

  • Kang, H.K.; Lee, S.S.
    Purification of the NADH reductase component of the steroid 9alpha-hydroxylase from Mycobacterium fortuitum (1997), Arch. Pharm. Res., 20, 590-596.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.99.24 medicine enzyme catalyzes the production of 9alpha-hydroxy-4-androstene-3,17-dione, an important intermediate for the semi-synthesis of potent anti-inflammatory drugs such as 9alpha-fluorocorticoids from 4-androstene-3,17-dione Mycolicibacterium fortuitum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.24 0.068
-
 NADH
-
 Mycolicibacterium fortuitum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.24 cytosol
-
 Mycolicibacterium fortuitum 5829
-
1.14.99.24 cytosol
-
 Nocardia sp. 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.24 Fe2+ iron-sulfur group Mycolicibacterium fortuitum
1.14.99.24 Fe2+
-
 Nocardia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.24 60000
-
 NADH reductase component, SDS-PAGE Mycolicibacterium fortuitum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Nocardia sp.
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Mycolicibacterium fortuitum
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.24 Mycolicibacterium fortuitum
-
 KCTC 1122, ATCC 6842
-
1.14.99.24 Nocardia sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.24 NADH reductase component Mycolicibacterium fortuitum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.24 6.18
-
 NADH reductase component Mycolicibacterium fortuitum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Nocardia sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Mycolicibacterium fortuitum 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.24 FAD
-
 Mycolicibacterium fortuitum
1.14.99.24 FAD
-
 Nocardia sp.
1.14.99.24 NADH
-
 Mycolicibacterium fortuitum
1.14.99.24 NADH
-
 Nocardia sp.