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Literature summary extracted from

  • Sonomoto K.; Usuin N.; Tanaka A.; Fukui S.
    9alpha-Hydroxylation of 4-androstene-3,17-dione by gel-entrapped Corynebacterium sp. cells (1983), Eur. J. Appl. Microbiol. Biotechnol., 17, 203-210.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.99.24 dimethyl sulfoxide reaction rate 111% Corynebacterium sp.
1.14.99.24 methanol reaction rate 111% Corynebacterium sp.
1.14.99.24 N,N-Dimethylformamide reaction rate 111% Corynebacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2 Corynebacterium sp.
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.24 Corynebacterium sp.
-
 synonym Nocardia restrictus, ATCC 14887 (MCI 1354)
-

Storage Stability

EC Number Storage Stability Organism
1.14.99.24 -20°C, free cells retain the original hydroxylation activity for at least 1 month Corynebacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.24 4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Corynebacterium sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
1.14.99.24 4-androstene-3,17-dione + NADH + O2
-
 Corynebacterium sp. 9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.99.24 20 30 entrapped cells Corynebacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.24 8
-
 entrapped cells Corynebacterium sp.
1.14.99.24 9
-
 free cells Corynebacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.99.24 6 9.5 entrapped cells Corynebacterium sp.