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Literature summary extracted from
- The steroid-9alpha-hydroxylation system from Nocardia species (1982), Eur. J. Biochem., 128, 125-135.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.99.24 | synthesis | enzyme occurs in many bacterial genera used in industrial processes | Corynebacterium sp. |
| 1.14.99.24 | synthesis | enzyme occurs in many bacterial genera used in industrial processes | Arthrobacter sp. |
| 1.14.99.24 | synthesis | enzyme occurs in many bacterial genera used in industrial processes | Mycobacterium sp. |
| 1.14.99.24 | synthesis | enzyme occurs in many bacterial genera used in industrial processes | Nocardia sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.24 | 2,2'-bipyridine | - |
Corynebacterium sp. |  |
| 1.14.99.24 | 2,2'-bipyridine | 52% inhibition | Nocardia sp. | |
| 1.14.99.24 | Cd(CH3COO)2 | 80% inhibition | Nocardia sp. | |
| 1.14.99.24 | CuSO4 | 100% inhibition | Nocardia sp. | |
| 1.14.99.24 | Hg(CH3COO)2 | 33% inhibition | Nocardia sp. | |
| 1.14.99.24 | o-phenanthroline | - |
Corynebacterium sp. | |
| 1.14.99.24 | o-phenanthroline | - |
Mycobacterium sp. | |
| 1.14.99.24 | o-phenanthroline | 72% inhibition | Nocardia sp. | |
| 1.14.99.24 | potassium cyanide | 60% inhibition | Nocardia sp. | |
| 1.14.99.24 | Sodium azide | 100% inhibition | Nocardia sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.24 | Fe2+ | iron-sulfur protein | Nocardia sp. | |
| 1.14.99.24 | Mg2+ | higher hydroxylation obtained by increasing magnesium ion concentration with a maximum of stimulation at 20 mM or more | Nocardia sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.24 | 120000 | - |
hydroxylase system composed of 3 proteins, protein III, comparative gel filtration | Nocardia sp. |
| 1.14.99.24 | 214000 | - |
hydroxylase system composed of 3 proteins, protein II, comparative gel filtration | Nocardia sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.24 | 4,9(11)-pregnadiene-3,20-dione + NADH + O2 | Corynebacterium sp. | - |
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O | - |
? | |
| 1.14.99.24 | 4,9(11)-pregnadiene-3,20-dione + NADH + O2 | Arthrobacter sp. | - |
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O | - |
? | |
| 1.14.99.24 | 4,9(11)-pregnadiene-3,20-dione + NADH + O2 | Mycobacterium sp. | - |
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O | - |
? | |
| 1.14.99.24 | 4,9(11)-pregnadiene-3,20-dione + NADH + O2 | Nocardia sp. | key-enzyme in the steroid-ring-B-splitting pathway | 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O | - |
? | |
| 1.14.99.24 | 4,9(11)-pregnadiene-3,20-dione + NADH + O2 | Nocardia sp. M117 | key-enzyme in the steroid-ring-B-splitting pathway | 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.24 | Arthrobacter sp. | - |
- |
- |
| 1.14.99.24 | Corynebacterium sp. | - |
- |
- |
| 1.14.99.24 | Mycobacterium sp. | - |
- |
- |
| 1.14.99.24 | Nocardia sp. | - |
- |
- |
| 1.14.99.24 | Nocardia sp. M117 | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.14.99.24 | protein II and protein III are oxygen-labile | Nocardia sp. |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.99.24 | partially | Nocardia sp. |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.99.24 | -100°C, enzyme activity in clear supernatant frozen in liquid nitrogen is stable for more than 1 year | Nocardia sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.24 | 4,9(11)-pregnadiene-3,20-dione + NADH + O2 | - |
Corynebacterium sp. | 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O | - |
? | |
| 1.14.99.24 | 4,9(11)-pregnadiene-3,20-dione + NADH + O2 | - |
Arthrobacter sp. | 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O | - |
? | |
| 1.14.99.24 | 4,9(11)-pregnadiene-3,20-dione + NADH + O2 | - |
Mycobacterium sp. | 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O | - |
? | |
| 1.14.99.24 | 4,9(11)-pregnadiene-3,20-dione + NADH + O2 | key-enzyme in the steroid-ring-B-splitting pathway | Nocardia sp. | 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O | - |
? | |
| 1.14.99.24 | 4,9(11)-pregnadiene-3,20-dione + NADH + O2 | key-enzyme in the steroid-ring-B-splitting pathway | Nocardia sp. M117 | 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.99.24 | More | enzyme system involves a flavoprotein reductase and 2 iron-sulfur proteins | Nocardia sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.24 | 9.5 | - |
- |
Nocardia sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.24 | 5.5 | 10 | - |
Nocardia sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.24 | FAD | flavoprotein | Nocardia sp. | |
| 1.14.99.24 | NADH | - |
Mycobacterium sp. | |
| 1.14.99.24 | NADH | - |
Nocardia sp. | |
| 1.14.99.24 | NADPH | conversion rate higher with NADH | Nocardia sp. | |
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