Literature summary extracted from
Wilks, A.; Schmitt, M.P.
Expression and characterization of a heme oxygenase (Hmu O) from Corynebacterium diphtheriae. Iron acquisition requires oxidative cleavage of the heme macrocyle (1998), J. Biol. Chem., 273, 837-841.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.14.18 |
expression of wild-type and His-tagged enzyme in Escherichia coli |
Corynebacterium diphtheriae |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.14.14.18 |
25000 |
- |
x * 25000, recombinant enzyme, SDS-PAGE |
Corynebacterium diphtheriae |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.14.18 |
Corynebacterium diphtheriae |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.14.18 |
recombinant wild-type and His-tagged enzyme, ion-exchange, gel filtration |
Corynebacterium diphtheriae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.14.18 |
heme + electron donor + O2 |
electron donor NADPH, reductase: human or E. coli NADPH-cytochrome P450 reductase or putidaredoxin/putidaredoxin reductase |
Corynebacterium diphtheriae |
biliverdin + Fe2+ + CO + oxidized eletron donor + H2O |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.14.18 |
? |
x * 25000, recombinant enzyme, SDS-PAGE |
Corynebacterium diphtheriae |