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Literature summary extracted from

  • Kutty, R.K.; Maines, M.D.
    Characterization of an NADH-dependent haem-degrading system in ox heart mitochondria (1987), Biochem. J., 246, 467-474.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.18 mitochondrion 77% activity in inner membrane, specifically associated with complex I, NADH: ubiquinone oxidoreductase Bos taurus 5739
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 additional information Rattus norvegicus microsomal heme oxygenase system composed of heme oxygenase and NADPH-cytochrome c reductase catalyzes the oxidative degradation of heme to biliverdin, essential role in the physiological heme catabolism ?
-
 ?
1.14.14.18 heme + 2 NADH + 2 H+ + 2 O2 Bos taurus NADH-dependent heme degradation system may have a biological role in regulating the concentration of respiratory hemoproteins and the disposition of the aberrant forms of the mitochondrial hemoproteins biliverdin + Fe2+ + CO + 2 NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Rattus norvegicus
-
-
-
1.14.14.18 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 liver
-
 Rattus norvegicus
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1.14.14.18 heart
-
 Bos taurus
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 30 50
-
 Rattus norvegicus
1.14.14.18 0.00046
-
 NADH-dependent heme degradation in heart mitochondria Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH
-
 Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 additional information microsomal heme oxygenase system composed of heme oxygenase and NADPH-cytochrome c reductase catalyzes the oxidative degradation of heme to biliverdin, essential role in the physiological heme catabolism Rattus norvegicus ?
-
 ?
1.14.14.18 heme + 2 NADH + 2 H+ + 2 O2 NADH-dependent heme degradation system may have a biological role in regulating the concentration of respiratory hemoproteins and the disposition of the aberrant forms of the mitochondrial hemoproteins Bos taurus biliverdin + Fe2+ + CO + 2 NAD+ + H2O
-
 ?
1.14.14.18 heme + electron donor + O2 electron donor NADH Bos taurus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
 ?
1.14.14.18 heme + electron donor + O2 NADH-dependent heme-degradation activity, 16% of NADH activity with 0.5 mM NADPH Bos taurus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
 ?
1.14.14.18 heme + electron donor + O2 cytochrome c and myoglobin are not oxidized Bos taurus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
 ?
1.14.14.18 heme + electron donor + O2 electron donor NADPH Bos taurus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.18 90
-
 15 min, 95% loss of activity, crude extract Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 NADPH
-
 Rattus norvegicus
1.14.14.18 NADPH 0.5 mM, 16% as effective as NADH Bos taurus