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Literature summary extracted from
- Purification and properties of bovine spleen heme oxygenase. Amino acid composition and sites of action of inhibitors of heme oxidation (1982), J. Biol. Chem., 257, 7778-7785.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.14.18 | 10-20% loss of activity upon thawing | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.18 | Co-protoporphyrin | - |
Bos taurus |  |
| 1.14.14.18 | Hg2+ | inhibition of NADPH-cytochrome c reductase or biliverdin reductase in reconstituted heme oxygenase system | Bos taurus | |
| 1.14.14.18 | Mn-protoporphyrin | - |
Bos taurus | |
| 1.14.14.18 | p-chloromercuribenzoate | inhibition of NADPH-cytochrome c reductase in reconstituted heme oxygenase system | Bos taurus | |
| 1.14.14.18 | Porphyrins | metalloporphyrins | Bos taurus | |
| 1.14.14.18 | Sn-protoporphyrin | - |
Bos taurus | |
| 1.14.14.18 | Zn-protoporphyrin | - |
Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.18 | 0.00093 | - |
heme | - |
Bos taurus | |
| 1.14.14.18 | 0.023 | - |
NADPH | - |
Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.18 | microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.18 | 31000 | - |
x * 31000, SDS-PAGE | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.18 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.18 | Triton X-100 + cholate, DEAE-cellulose, hydroxyapatite, Sepharose CL-6B, hydroxyapatite | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.18 | spleen | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.18 | 0.113 | - |
spleen enzyme | Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.18 | 4°C, 20 mM potassium phosphate, pH 7.5, 0.2% Triton X-100, 20% glycerol, 2 d, 50% loss of activity | Bos taurus |
| 1.14.14.18 | liquid nitrogen, 6 months, no loss of activity | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.18 | heme + 2 NADPH + 2 H+ + 2 O2 | - |
Bos taurus | biliverdin + Fe2+ + CO + 2 NADP+ + H2O | - |
? | |
| 1.14.14.18 | heme + electron donor + O2 | NADH can replace NADPH at concentrations higher than 5 mM in vitro, NADH is unlikely to be an electron donor in vivo | Bos taurus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
| 1.14.14.18 | heme + electron donor + O2 | electron donor NADPH | Bos taurus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.18 | ? | x * 31000, SDS-PAGE | Bos taurus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.18 | 0.0583 | - |
heme | - |
Bos taurus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.18 | 0.000033 | - |
Sn-protoporphyrin | - |
Bos taurus | |
| 1.14.14.18 | 0.000082 | - |
Co-protoporphyrin | - |
Bos taurus | |
| 1.14.14.18 | 0.00013 | - |
Zn-protoporphyrin | - |
Bos taurus | |
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