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Literature summary extracted from

  • Rahim, M.A.; Sih, C.J.
    Mechanisms of steroid oxidation by microorganisms. XI. Enzymatic cleavage of the pregnane side chain (1966), J. Biol. Chem., 241, 3615-3623.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.99.4 1 mg/ml albumin stabilizes crude enzyme extract for 1 month when kept frozen Ilyonectria destructans
1.14.99.4 10 h dialysis of purified enzyme against 0.01 M Tris buffer, pH 7.5, 0.005 M EDTA, leads to 50% loss of activity Ilyonectria destructans
1.14.99.4 EDTA affords some protection, especially for crude extracts Ilyonectria destructans
1.14.99.4 preparation is highly unstable Ilyonectria destructans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.4 KCN
-
Ilyonectria destructans
1.14.99.4 methylene blue
-
Ilyonectria destructans
1.14.99.4 p-hydroxymercuribenzoate
-
Ilyonectria destructans
1.14.99.4 phenazine methosulfate
-
Ilyonectria destructans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.4 progesterone + NADPH + H+ + O2 Amorphotheca resinae
-
Testosterone acetate + NADP+ + H2O
-
?
1.14.99.4 progesterone + NADPH + H+ + O2 Ilyonectria destructans
-
Testosterone acetate + NADP+ + H2O
-
?
1.14.99.4 progesterone + NADPH + H+ + O2 Ilyonectria destructans ACC 11011
-
Testosterone acetate + NADP+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.4 Amorphotheca resinae
-
-
-
1.14.99.4 Ilyonectria destructans
-
ACC 11011
-
1.14.99.4 Ilyonectria destructans ACC 11011
-
ACC 11011
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.4
-
Ilyonectria destructans

Storage Stability

EC Number Storage Stability Organism
1.14.99.4 0-6°, 85% of the enzyme activity is lost in 24 h Ilyonectria destructans
1.14.99.4 0°C, 80% enzyme activity is lost in 7 days, half-life of a highly purified preparation is 12 h Ilyonectria destructans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.4 16alpha,17alpha-oxidopregn-4-ene-3,20-dione + NADPH + O2 degradation of pregnane side chains Ilyonectria destructans 16alpha-hydroxyandrost-4-ene-3,17-dione
-
?
1.14.99.4 16alpha,17alpha-oxidopregn-4-ene-3,20-dione + NADPH + O2 degradation of pregnane side chains Ilyonectria destructans ACC 11011 16alpha-hydroxyandrost-4-ene-3,17-dione
-
?
1.14.99.4 17alpha-hydroxypregn-4-ene-3,20-dione + NADPH + O2 degradation of pregnane side chains, about 210% the rate of the reaction with progesterone Ilyonectria destructans androst-4-ene-3,17-dione + NADP+ + ?
-
?
1.14.99.4 17alpha-hydroxypregn-4-ene-3,20-dione + NADPH + O2 degradation of pregnane side chains, about 210% the rate of the reaction with progesterone Ilyonectria destructans ACC 11011 androst-4-ene-3,17-dione + NADP+ + ?
-
?
1.14.99.4 17alpha-methylpregn-4-ene-3,20-dione + NADPH + O2 about 4.3% the rate of the reaction with progesterone Ilyonectria destructans ?
-
?
1.14.99.4 deoxycorticosterone + NADPH + O2 degradation of pregnane side chains, about 64% the rate of the reaction with progesterone Ilyonectria destructans testosterone + NADP+ + ?
-
?
1.14.99.4 deoxycorticosterone + NADPH + O2 degradation of pregnane side chains, about 64% the rate of the reaction with progesterone Ilyonectria destructans ACC 11011 testosterone + NADP+ + ?
-
?
1.14.99.4 additional information cortisol, 20alpha-hydroxy-16alpha,17alpha-oxidopregn-4-ene-3,20-dione and 20beta-hydroxypregn-4-ene-3-one are no substrates Ilyonectria destructans ?
-
?
1.14.99.4 additional information cortisol, 20alpha-hydroxy-16alpha,17alpha-oxidopregn-4-ene-3,20-dione and 20beta-hydroxypregn-4-ene-3-one are no substrates Ilyonectria destructans ACC 11011 ?
-
?
1.14.99.4 pregna-4,16-diene-3,20-dione + NADPH + O2 degradation of pregnane side chains, about 104% the rate of the reaction with progesterone Ilyonectria destructans androst-4-ene-3,17-dione
-
?
1.14.99.4 progesterone + NADPH + H+ + O2
-
Amorphotheca resinae Testosterone acetate + NADP+ + H2O
-
?
1.14.99.4 progesterone + NADPH + H+ + O2
-
Ilyonectria destructans Testosterone acetate + NADP+ + H2O
-
?
1.14.99.4 progesterone + NADPH + H+ + O2
-
Ilyonectria destructans ACC 11011 Testosterone acetate + NADP+ + H2O
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.4 7.5
-
progesterone, deoxycorticosterone, 17alpha-hydroxypregn-4-ene-3,20-dione as substrates Ilyonectria destructans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.99.4 5.4 9 irreversible inactivated below Ilyonectria destructans
1.14.99.4 7 8 most stable Ilyonectria destructans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.4 NADPH absolute requirement, FMN, FAD, NADH or 2-amino-6,7-dimethyl-4-hydroxy-5,6,7,8-tetrahydropteridine cannot replace NADPH as hydrogen-donor Ilyonectria destructans