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Literature summary extracted from

  • Abe, I.; Seki, T.; Umehara, K.; Miyase, T.; Noguchi, H.; Sakakibara, J.; Ono, T.
    Green tea polyphenols: novel and potent inhibitors of squalene epoxidase (2000), Biochem. Biophys. Res. Commun., 268, 767-771.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.14.17 medicine cholesterol lowering effect of green tea may be attributed to the enzyme inhibitory activities of its gallocatechins Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.17
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.17 (-)-epicatechin-3-O-gallate 50% inhibition at 0.0013 mM Rattus norvegicus
1.14.14.17 (-)-epigallocatechin-3-O-gallate 50% inhibition at 0.00069 mM, noncompetitive Rattus norvegicus
1.14.14.17 (-)-gallocatechin-3-O-gallate 50% inhibition at 0.00067 mM Rattus norvegicus
1.14.14.17 epigallocatechin-3-O-gallate
-
 Rattus norvegicus
1.14.14.17 additional information cholesterol lowering effect of green tea gallocatechins may be attributed to their potent squalene oxidase inhibition; not inhibitory: flavan-3-ols with galloyl group at C-3 Rattus norvegicus
1.14.14.17 theasinensin A 50% inhibition at 0.00013 mM Rattus norvegicus
1.14.14.17 trisnorsqualene alcohol 0.004 mM, 50% inhibition Sus scrofa
1.14.14.17 trisnorsqualene cyclopropylamine 0.002 mM, 50% inhibition Sus scrofa
1.14.14.17 trisnorsqualene difluoromethylidene 0.0054 mM, 50% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.17 0.0036
-
 squalene
-
 Rattus norvegicus
1.14.14.17 0.0038
-
 squalene 37°C, pH 7.4 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.17 Rattus norvegicus
-
-
-
1.14.14.17 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.17
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.17 squalene + [reduced NADPH-hemoprotein reductase] + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + [oxidized NADPH-hemoprotein reductase] + H2O proposed mechanism Rattus norvegicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.17 squalene + electron donor + O2
-
 Rattus norvegicus (S)-squalene-2,3-epoxide + oxidized electron donor + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.17 0.0683
-
 squalene N-terminal trucated recombinant enzyme Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.14.17 0.00074
-
 epigallocatechin-3-O-gallate non-competitive, non-time dependent inhibition Rattus norvegicus
1.14.14.17 0.00074
-
 (-)-epigallocatechin-3-O-gallate 37°C, pH 7.4 Rattus norvegicus