Literature summary extracted from
Carter, C.; Thornburg, R.W.
Tobacco nectarin I. Purification and characterization as a germin-like, manganese superoxide dismutase implicated in the defense of floral reproductive tissues (2000), J. Biol. Chem., 275, 36726-36733.
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.15.1.1 |
CN- |
no inhibition Mn-SOD |
Nicotiana sp. |
|
1.15.1.1 |
H2O2 |
no inhibition: Mn-SOD |
Nicotiana sp. |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.15.1.1 |
extracellular |
Mn-SOD, nectar |
Nicotiana sp. |
- |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.15.1.1 |
Mn2+ |
specific for |
Nicotiana sp. |
|
1.15.1.1 |
Mn2+ |
0.5 mol per mol of subunit |
Nicotiana sp. |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.15.1.1 |
165000 |
- |
nectarin I: Mn-SOD, native PAGE |
Nicotiana sp. |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.15.1.1 |
Nicotiana sp. |
- |
nectarin I with Mn-SOD activity |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
1.15.1.1 |
glycoprotein |
biantennary nonasaccharide with composition (GlnNAc)4:Man3:Xyl:Fuc |
Nicotiana sp. |
1.15.1.1 |
glycoprotein |
nectarin I: Mn-SOD |
Nicotiana sp. |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.15.1.1 |
nectarin I: Mn-SOD from nectar |
Nicotiana sp. |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
1.15.1.1 |
activity cannot be restored by Fe2+, Cu2+, Zn2+, and Cu2+/Zn2+, but by Mn2+ |
Nicotiana sp. |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.15.1.1 |
nectar |
nectarin I |
Nicotiana sp. |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.15.1.1 |
additional information |
- |
- |
Nicotiana sp. |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.15.1.1 |
O2- + H+ |
- |
Nicotiana sp. |
O2 + H2O2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.15.1.1 |
? |
x * 22500-29000, nectarin I: Mn-SOD, SDS-PAGE and mass spectroscopy |
Nicotiana sp. |
1.15.1.1 |
More |
enzyme is only active as tetramer or pentamer |
Nicotiana sp. |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.15.1.1 |
nectarin I |
- |
Nicotiana sp. |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
1.15.1.1 |
90 |
- |
nectarin I: Mn-SOD, 85% remaining activity after 1 h |
Nicotiana sp. |
1.15.1.1 |
100 |
- |
complete loss of activity |
Nicotiana sp. |