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Literature summary extracted from

  • Santos, R.; Bocquet, S.; Puppo, A.; Touati, D.
    Characterization of an atypical superoxide dismutase from Sinorhizobium meliloti (1999), J. Bacteriol., 181, 4509-4516.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 atypical SOD, functional and structural intermediate between Fe-SOD and Mn-SOD, expression in Escherichia coli Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2 no inhibition: Fe-SOD; no inhibition: Mn-SOD Sinorhizobium meliloti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Iron 0.24 mol Fe2+ per mol of subunit Sinorhizobium meliloti
1.15.1.1 Mn2+ 0.75 mol per mol of subunit Sinorhizobium meliloti
1.15.1.1 Mn2+ accepts iron and/or manganese as cofactor Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 23000
-
2 * 23000, Fe-/Mn-SOD, SDS-PAGE Sinorhizobium meliloti
1.15.1.1 43000
-
Fe-/Mn-SOD, gel filtration Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Sinorhizobium meliloti
-
-
-
1.15.1.1 Sinorhizobium meliloti
-
structural intermediate between Mn-SOD and Fe-SOD
-
1.15.1.1 Sinorhizobium meliloti Rm5000
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 structural intermediate between Mn-SOD and Fe-SOD Sinorhizobium meliloti

Reaction

EC Number Reaction Comment Organism Reaction ID
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 amino acid sequence alignment and comparison Sinorhizobium meliloti

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.15.1.1 reconstitution of active protein after withdrawal of metal, higher activity with Mn2+, lower activity with Fe3+ Sinorhizobium meliloti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 additional information
-
native and recombinant, wild-type and restored enzyme, with Fe2+ and Mn2+ Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2- + H+
-
Sinorhizobium meliloti O2 + H2O2
-
?
1.15.1.1 O2- + H+
-
Sinorhizobium meliloti Rm5000 O2 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer 2 * 23000, Fe-/Mn-SOD, SDS-PAGE Sinorhizobium meliloti