EC Number | Cloned (Comment) | Organism |
---|---|---|
1.15.1.1 | atypical SOD, functional and structural intermediate between Fe-SOD and Mn-SOD, expression in Escherichia coli | Sinorhizobium meliloti |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | H2O2 | no inhibition: Fe-SOD; no inhibition: Mn-SOD | Sinorhizobium meliloti |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | Iron | 0.24 mol Fe2+ per mol of subunit | Sinorhizobium meliloti | |
1.15.1.1 | Mn2+ | 0.75 mol per mol of subunit | Sinorhizobium meliloti | |
1.15.1.1 | Mn2+ | accepts iron and/or manganese as cofactor | Sinorhizobium meliloti |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 23000 | - |
2 * 23000, Fe-/Mn-SOD, SDS-PAGE | Sinorhizobium meliloti |
1.15.1.1 | 43000 | - |
Fe-/Mn-SOD, gel filtration | Sinorhizobium meliloti |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.15.1.1 | Sinorhizobium meliloti | - |
- |
- |
1.15.1.1 | Sinorhizobium meliloti | - |
structural intermediate between Mn-SOD and Fe-SOD | - |
1.15.1.1 | Sinorhizobium meliloti Rm5000 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.15.1.1 | structural intermediate between Mn-SOD and Fe-SOD | Sinorhizobium meliloti |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.15.1.1 | 2 superoxide + 2 H+ = O2 + H2O2 | amino acid sequence alignment and comparison | Sinorhizobium meliloti |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.15.1.1 | reconstitution of active protein after withdrawal of metal, higher activity with Mn2+, lower activity with Fe3+ | Sinorhizobium meliloti |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | additional information | - |
native and recombinant, wild-type and restored enzyme, with Fe2+ and Mn2+ | Sinorhizobium meliloti |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | O2- + H+ | - |
Sinorhizobium meliloti | O2 + H2O2 | - |
? | |
1.15.1.1 | O2- + H+ | - |
Sinorhizobium meliloti Rm5000 | O2 + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.15.1.1 | dimer | 2 * 23000, Fe-/Mn-SOD, SDS-PAGE | Sinorhizobium meliloti |