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Literature summary extracted from

  • An, S.S.; Kim, Y.M.
    Purification and characterization of a manganese-containing superoxide dismutase from a carboxydobacterium, Pseudomonas carboxydohydrogena (1997), Mol. Cells, 7, 730-737.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 CN- no inhibition Pseudomonas carboxydohydrogena
1.15.1.1 H2O2 no inhibition: Mn-SOD Pseudomonas carboxydohydrogena
1.15.1.1 N3- Mn-SOD Pseudomonas carboxydohydrogena

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Mn2+ 1.1 mol per mol of enzyme Pseudomonas carboxydohydrogena

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 21700
-
 2 * 21700, Mn-SOD, SDS-PAGE Pseudomonas carboxydohydrogena
1.15.1.1 42500
-
 Mn-SOD, gel filtration Pseudomonas carboxydohydrogena

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Pseudomonas carboxydohydrogena
-
 Mn-SOD
-
1.15.1.1 Pseudomonas carboxydohydrogena
-
 strain DSM 1083
-
1.15.1.1 Pseudomonas carboxydohydrogena Mn-SOD
-
 Mn-SOD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 Mn-SOD Pseudomonas carboxydohydrogena

Reaction

EC Number Reaction Comment Organism Reaction ID
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 amino acid composition, comparison Pseudomonas carboxydohydrogena
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 additional information
-
-
 Pseudomonas carboxydohydrogena

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2- + H+
-
 Pseudomonas carboxydohydrogena O2 + H2O2
-
 ?
1.15.1.1 O2- + H+
-
 Pseudomonas carboxydohydrogena Mn-SOD O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer 2 * 21700, Mn-SOD, SDS-PAGE Pseudomonas carboxydohydrogena

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 25
-
 assay at Pseudomonas carboxydohydrogena

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 50
-
 88.5% remaining activity after 1 h Pseudomonas carboxydohydrogena
1.15.1.1 70
-
 complete loss of activity after 40 min Pseudomonas carboxydohydrogena
1.15.1.1 90
-
 complete loss of activity after 5 min Pseudomonas carboxydohydrogena

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 9
-
 Mn-SOD Pseudomonas carboxydohydrogena