EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | 8-Hydroxyquinoline-5-sulfonate | Fe-SOD, slightly stimulating, depending on the assay method | Pseudomonas putida | |
1.15.1.1 | o-phenanthroline | Fe-SOD, slightly stimulating | Pseudomonas putida | |
1.15.1.1 | o-phenanthroline | depending on assay method | Pseudomonas putida |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.15.1.1 | Fe-SOD, dialysis against 55% saturated ammonium sulfate solution, pH 4.5, 1 week at 2°C under reduced pressure | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | EDTA | no inhibition | Pseudomonas putida | |
1.15.1.1 | H2O2 | Fe-SOD | Pseudomonas putida | |
1.15.1.1 | additional information | no inhibition by N-ethylmaleide | Pseudomonas putida | |
1.15.1.1 | o-phenanthroline | depending on assay method; Fe-SOD | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | Iron | 1.0-1.45 mol per mol of enzyme | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 19500 | - |
2 * 19500, Fe-SOD, SDS-PAGE | Pseudomonas putida |
1.15.1.1 | 40000 | - |
Fe-SOD, gel filtration, sedimentation analysis | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.15.1.1 | Pseudomonas putida | - |
Fe-SOD | - |
1.15.1.1 | Pseudomonas putida Fe-SOD | - |
Fe-SOD | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.15.1.1 | Fe-SOD | Pseudomonas putida |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.15.1.1 | 2 superoxide + 2 H+ = O2 + H2O2 | amino acid composition, comparison | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | additional information | - |
- |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | O2- + H+ | - |
Pseudomonas putida | O2 + H2O2 | - |
? | |
1.15.1.1 | O2- + H+ | - |
Pseudomonas putida Fe-SOD | O2 + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.15.1.1 | dimer | 2 * 19500, Fe-SOD, SDS-PAGE | Pseudomonas putida |