Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reinards, R.; Altdorf, R.; Ohlenbusch, H.D.
    Purification and properties of a manganese-containing superoxide dismutase from Acholeplasma laidlawii (1984), Hoppe-Seyler's Z. Physiol. Chem., 365, 577-585.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.15.1.1 lyophilization: less than 10% loss of activity Acholeplasma laidlawii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2 no inhibition: Mn-SOD Acholeplasma laidlawii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Mn2+ 0.5 mol per mol of subunit Acholeplasma laidlawii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 21600
-
 2 * 21600, SDS-PAGE after denaturation in boiling SDS Acholeplasma laidlawii
1.15.1.1 41500
-
 gel filtration Acholeplasma laidlawii
1.15.1.1 41500
-
 Mn-SOD Acholeplasma laidlawii

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Acholeplasma laidlawii
-
 Mn-SOD
-
1.15.1.1 Acholeplasma laidlawii Mn-SOD
-
 Mn-SOD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 Mn-SOD Acholeplasma laidlawii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 amino acid composition, comparison Acholeplasma laidlawii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 additional information
-
-
 Acholeplasma laidlawii

Storage Stability

EC Number Storage Stability Organism
1.15.1.1 4°C, 10 mM phosphate buffer, pH 7.2, 4 weeks Acholeplasma laidlawii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2- + H+
-
 Acholeplasma laidlawii O2 + H2O2
-
 ?
1.15.1.1 O2- + H+
-
 Acholeplasma laidlawii Mn-SOD O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer 2 * 21600, SDS-PAGE after denaturation in boiling SDS Acholeplasma laidlawii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 40
-
 1 h, about 20% loss of activity Acholeplasma laidlawii
1.15.1.1 50
-
 1 h, about 55% loss of activity Acholeplasma laidlawii
1.15.1.1 55
-
 10 min, about 45% loss of activity, 30 min, 75% loss of activity Acholeplasma laidlawii
1.15.1.1 60
-
 10 min, about 75% loss of activity Acholeplasma laidlawii