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Literature summary extracted from

  • Mirande, M.; Le Corre, D.; Waller, J.P.
    A complex from cultured Chinese hamster ovary cells containing nine aminoacyl-tRNA synthetases. Thermolabile leucyl-tRNA synthetase from the tsH1 mutant cell line is an integral component of this complex (1985), Eur. J. Biochem., 147, 281-289.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.4 tsH1 temperature sensitive mutant tsH1 Cricetulus griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 129000
-
 x * 129000 + x * ?, SDS-PAGE, the 129000 MW polypeptide is a component of the high-MW aminoacyl-tRNA synthetase complex (MW 1200000, gel filtration) Cricetulus griseus
6.1.1.4 1200000
-
 containing 9 amino acyl-tRNA synthetases specific for Glu, Pro, Ile, Leu, Gln, Lys, Arg and Asp Cricetulus griseus
6.1.1.4 1200000
-
 gel filtration, multienzyme complex Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Cricetulus griseus
-
 high-MW enzyme complex
-
6.1.1.4 Cricetulus griseus
-
 temperature-sensitive mutant tsH1
-
6.1.1.4 Cricetulus griseus
-
 wild-type
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4
-
 Cricetulus griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.4 0.0068
-
-
 Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Cricetulus griseus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 ? x * 129000 + x * ?, SDS-PAGE, the 129000 MW polypeptide is a component of the high-MW aminoacyl-tRNA synthetase complex (MW 1200000, gel filtration) Cricetulus griseus