Literature summary extracted from

Rova, U.; Adrait, A.; Potsch, S.; Graslund, A.; Thelander, L.
Evidence by mutagenesis that Tyr370 of the mouse ribonucleotide reductase R2 protein is the connecting link in the intersubunit radical transfer pathway (1999), J. Biol. Chem., 274, 23746-23751.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.17.4.1	expression of Y370F and Y370W mutant enzymes in Escherichia coli	Mus musculus

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.17.4.1	Y370F	mutation in R2 subunit, no activity	Mus musculus
1.17.4.1	Y370W	mutation in R2 subunit, point mutation does not affect the ability to form a normal diferric iron/tyrosyl radical center, 1.7% of wild-type activity probably due to slow radical transfer	Mus musculus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.17.4.1	Mus musculus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.17.4.1	Y370F and Y370w mutant enzymes	Mus musculus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.17.4.1	ribonucleoside diphosphate + reduced thioredoxin	-	Mus musculus	2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O	-	ir

Subunits

EC Number	Subunits	Comment	Organism
1.17.4.1	More	large subunit R1 contains binding sites for substrates and allosteric effectors, smaller subunit R2 contains non-heme iron and a tyrosyl free-radical	Mus musculus

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Release 2023.1 (January 2023)