BRENDA - Enzyme Database

High-performance liquid chromatographic purification, optimization of the assay, and properties of ribonucleoside diphosphate reductase from rabbit bone marrow

Sinhababu, A.K.; Boehlert, C.C.; Gan, L.S.; Yanni, S.B.; Thakker, D.R.; Arch. Biochem. Biophys. 317, 285-291 (1995)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
2-mercaptoethanol
25 mM, maximal activation, 70% of activity with dithiothreitol
Oryctolagus cuniculus
1.17.4.1
dithioerythritol
0.5 mM, maximal activation, 94% of activity with dithiothreitol
Oryctolagus cuniculus
1.17.4.1
dithiothreitol
optimal in vitro activity with 10 mM, inhibition above
Oryctolagus cuniculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
dithiothreitol
higher than 10 mM, activation below
Oryctolagus cuniculus
1.17.4.1
Hydroxyurea
-
Oryctolagus cuniculus
1.17.4.1
UDP
inhibition of CDP reduction
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.4.1
0.0014
-
CDP
in the presence of 5'-adenylimidodiphosphate
Oryctolagus cuniculus
1.17.4.1
0.0023
-
CDP
in the presence of ATP
Oryctolagus cuniculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.4.1
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.4.1
gel filtration, ATP-agarose, partial purification
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.4.1
0.0000045
-
partially purified enzyme, CDP reduction
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
-
437977
Oryctolagus cuniculus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437977
Oryctolagus cuniculus
ir
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.17.4.1
0.029
-
UDP
-
Oryctolagus cuniculus
1.17.4.1
0.69
-
Hydroxyurea
-
Oryctolagus cuniculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
2-mercaptoethanol
25 mM, maximal activation, 70% of activity with dithiothreitol
Oryctolagus cuniculus
1.17.4.1
dithioerythritol
0.5 mM, maximal activation, 94% of activity with dithiothreitol
Oryctolagus cuniculus
1.17.4.1
dithiothreitol
optimal in vitro activity with 10 mM, inhibition above
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
dithiothreitol
higher than 10 mM, activation below
Oryctolagus cuniculus
1.17.4.1
Hydroxyurea
-
Oryctolagus cuniculus
1.17.4.1
UDP
inhibition of CDP reduction
Oryctolagus cuniculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.17.4.1
0.029
-
UDP
-
Oryctolagus cuniculus
1.17.4.1
0.69
-
Hydroxyurea
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.4.1
0.0014
-
CDP
in the presence of 5'-adenylimidodiphosphate
Oryctolagus cuniculus
1.17.4.1
0.0023
-
CDP
in the presence of ATP
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.4.1
gel filtration, ATP-agarose, partial purification
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.4.1
0.0000045
-
partially purified enzyme, CDP reduction
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
-
437977
Oryctolagus cuniculus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437977
Oryctolagus cuniculus
ir