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Literature summary extracted from

  • Jordan, A.; Pontis, E.; Atta, M.; Krook, M.; Gibert, I.; Barbe, J.; Reichard, P.
    A second class I ribonucleotide reductase in Enterobacteriaceae: characterization of the Salmonella typhimurium enzyme (1994), Proc. Natl. Acad. Sci. USA, 91, 12892-12896.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 dATP
-
 Escherichia coli
1.17.4.1 dATP strong stimulation of CDP reduction Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1 dGTP
-
 Escherichia coli
1.17.4.1 dGTP slight stimulation of CDP reduction Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1 dTTP
-
 Escherichia coli
1.17.4.1 dTTP slight stimulation of CDP reduction Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 Hydroxyurea
-
 Escherichia coli
1.17.4.1 Hydroxyurea approx. 0.5 mM, 50% inhibition Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.1 0.00013
-
 glutaredoxin
-
 Escherichia coli
1.17.4.1 0.003
-
 dATP positive effector of CDP reduction Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1 0.006
-
 glutaredoxin 1
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1 0.016
-
 CDP
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Iron
-
 Escherichia coli
1.17.4.1 Iron 1.8 mol of iron per mol of R2F subunit, dinuclear iron center Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1 Mg2+
-
 Escherichia coli
1.17.4.1 Mg2+ activity depends on Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.1 36000
-
 alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1 70000
-
 alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Escherichia coli
-
-
-
1.17.4.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
 class I ribonucleotide reductase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 R1E subunit, ammonium sulfate, DEAE-Sepharose, dATP-Sepharose Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1 R2F subunit, ammonium sulfate, DEAE-Sepharose, Mono Q, Superdex Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.1 0.28
-
 CDP reduction of subunit R1E in the presence of subunit R2F Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1 0.83
-
 CDP reduction of subunit R2F in the presence of subunit R1E Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 CDP + reduced glutaredoxin 1
-
 Salmonella enterica subsp. enterica serovar Typhimurium dCDP + oxidized glutaredoxin 1 + H2O
-
 r
1.17.4.1 CDP + thioredoxin
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2'-dCDP + thioredoxin disulfide + H2O
-
 r
1.17.4.1 ribonucleoside diphosphate + reduced glutaredoxin
-
 Escherichia coli 2'-deoxyribonucleoside diphosphate + oxidized glutaredoxin + H2O
-
 ir
1.17.4.1 ribonucleoside diphosphate + reduced glutaredoxin 1
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2'-deoxyribonucleoside diphosphate + oxidized glutaredoxin 1 + H2O
-
 ir
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
 Escherichia coli 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin dithiothreitol serves as in vitro electron donor, maximal activity with 40 mM Salmonella enterica subsp. enterica serovar Typhimurium 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir

Subunits

EC Number Subunits Comment Organism
1.17.4.1 tetramer
-
 Escherichia coli
1.17.4.1 tetramer alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 8
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium