BRENDA - Enzyme Database

A second class I ribonucleotide reductase in Enterobacteriaceae: characterization of the Salmonella typhimurium enzyme

Jordan, A.; Pontis, E.; Atta, M.; Krook, M.; Gibert, I.; Barbe, J.; Reichard, P.; Proc. Natl. Acad. Sci. USA 91, 12892-12896 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
dATP
strong stimulation of CDP reduction
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
dATP
-
Escherichia coli
1.17.4.1
dGTP
-
Escherichia coli
1.17.4.1
dGTP
slight stimulation of CDP reduction
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
dTTP
-
Escherichia coli
1.17.4.1
dTTP
slight stimulation of CDP reduction
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
Hydroxyurea
-
Escherichia coli
1.17.4.1
Hydroxyurea
approx. 0.5 mM, 50% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.4.1
0.00013
-
glutaredoxin
-
Escherichia coli
1.17.4.1
0.003
-
dATP
positive effector of CDP reduction
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
0.006
-
glutaredoxin 1
-
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
0.016
-
CDP
-
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.4.1
Iron
-
Escherichia coli
1.17.4.1
Iron
1.8 mol of iron per mol of R2F subunit, dinuclear iron center
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
Mg2+
activity depends on Mg2+
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
Mg2+
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.4.1
36000
-
alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
70000
-
alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.4.1
Escherichia coli
-
-
-
1.17.4.1
Salmonella enterica subsp. enterica serovar Typhimurium
-
class I ribonucleotide reductase
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.4.1
R1E subunit, ammonium sulfate, DEAE-Sepharose, dATP-Sepharose; R2F subunit, ammonium sulfate, DEAE-Sepharose, Mono Q, Superdex
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.4.1
0.28
-
CDP reduction of subunit R1E in the presence of subunit R2F
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
0.83
-
CDP reduction of subunit R2F in the presence of subunit R1E
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced glutaredoxin
-
437976
Escherichia coli
2'-deoxyribonucleoside diphosphate + oxidized glutaredoxin + H2O
-
437976
Escherichia coli
ir
1.17.4.1
ribonucleoside diphosphate + reduced glutaredoxin 1
-
437976
Salmonella enterica subsp. enterica serovar Typhimurium
2'-deoxyribonucleoside diphosphate + oxidized glutaredoxin 1 + H2O
-
437976
Salmonella enterica subsp. enterica serovar Typhimurium
ir
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
-
437976
Escherichia coli
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437976
Escherichia coli
ir
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
dithiothreitol serves as in vitro electron donor, maximal activity with 40 mM
437976
Salmonella enterica subsp. enterica serovar Typhimurium
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437976
Salmonella enterica subsp. enterica serovar Typhimurium
ir
Subunits
EC Number
Subunits
Commentary
Organism
1.17.4.1
tetramer
-
Escherichia coli
1.17.4.1
tetramer
alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.1
8
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
dATP
strong stimulation of CDP reduction
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
dATP
-
Escherichia coli
1.17.4.1
dGTP
-
Escherichia coli
1.17.4.1
dGTP
slight stimulation of CDP reduction
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
dTTP
-
Escherichia coli
1.17.4.1
dTTP
slight stimulation of CDP reduction
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
Hydroxyurea
-
Escherichia coli
1.17.4.1
Hydroxyurea
approx. 0.5 mM, 50% inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.4.1
0.00013
-
glutaredoxin
-
Escherichia coli
1.17.4.1
0.003
-
dATP
positive effector of CDP reduction
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
0.006
-
glutaredoxin 1
-
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
0.016
-
CDP
-
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.4.1
Iron
-
Escherichia coli
1.17.4.1
Iron
1.8 mol of iron per mol of R2F subunit, dinuclear iron center
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
Mg2+
activity depends on Mg2+
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
Mg2+
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.4.1
36000
-
alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
70000
-
alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.4.1
R1E subunit, ammonium sulfate, DEAE-Sepharose, dATP-Sepharose; R2F subunit, ammonium sulfate, DEAE-Sepharose, Mono Q, Superdex
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.4.1
0.28
-
CDP reduction of subunit R1E in the presence of subunit R2F
Salmonella enterica subsp. enterica serovar Typhimurium
1.17.4.1
0.83
-
CDP reduction of subunit R2F in the presence of subunit R1E
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced glutaredoxin
-
437976
Escherichia coli
2'-deoxyribonucleoside diphosphate + oxidized glutaredoxin + H2O
-
437976
Escherichia coli
ir
1.17.4.1
ribonucleoside diphosphate + reduced glutaredoxin 1
-
437976
Salmonella enterica subsp. enterica serovar Typhimurium
2'-deoxyribonucleoside diphosphate + oxidized glutaredoxin 1 + H2O
-
437976
Salmonella enterica subsp. enterica serovar Typhimurium
ir
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
-
437976
Escherichia coli
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437976
Escherichia coli
ir
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
dithiothreitol serves as in vitro electron donor, maximal activity with 40 mM
437976
Salmonella enterica subsp. enterica serovar Typhimurium
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437976
Salmonella enterica subsp. enterica serovar Typhimurium
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.4.1
tetramer
-
Escherichia coli
1.17.4.1
tetramer
alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.1
8
-
-
Salmonella enterica subsp. enterica serovar Typhimurium