Literature summary extracted from
Slabaugh, M.B.; Davis, R.E.; Roseman, N.A.; Mathews, C.K.
Vaccinia virus ribonucleotide reductase expression and isolation of the recombinant large subunit (1993), J. Biol. Chem., 268, 17803-17810.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.17.4.1 |
adenyl-5'-yl-imidodiphosphate |
stimulation at low concentration, inhibition above 0.3 mM |
Vaccinia virus |
|
1.17.4.1 |
ATP |
most effective activator |
Vaccinia virus |
|
1.17.4.1 |
P1,P4-bis(5'-adenosyl) tetraphosphate |
stimulation at low concentrations, inhibition above 0.3 mM |
Vaccinia virus |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.17.4.1 |
expression in Escherichia coli |
Vaccinia virus |
General Stability
EC Number |
General Stability |
Organism |
---|
1.17.4.1 |
quite susceptible to denaturation |
Vaccinia virus |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.17.4.1 |
Mg2+ |
2fold activation |
Vaccinia virus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.17.4.1 |
Vaccinia virus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.17.4.1 |
ammonium sulfate, Blue Sepharose, affinity resin |
Vaccinia virus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.17.4.1 |
0.122 |
- |
recombinant enzyme |
Vaccinia virus |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.17.4.1 |
-80°C, several months, no loss of activity |
Vaccinia virus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.17.4.1 |
ribonucleoside diphosphate + reduced thioredoxin |
dithiothreitol serves as in vitro electron donor, maximal activity with 50-75 mM |
Vaccinia virus |
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O |
- |
ir |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.17.4.1 |
8 |
8.8 |
- |
Vaccinia virus |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
1.17.4.1 |
6.8 |
8.8 |
60% activity at pH 7.2 |
Vaccinia virus |