BRENDA - Enzyme Database

Vaccinia virus ribonucleotide reductase expression and isolation of the recombinant large subunit

Slabaugh, M.B.; Davis, R.E.; Roseman, N.A.; Mathews, C.K.; J. Biol. Chem. 268, 17803-17810 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
adenyl-5'-yl-imidodiphosphate
stimulation at low concentration, inhibition above 0.3 mM
Vaccinia virus
1.17.4.1
ATP
most effective activator
Vaccinia virus
1.17.4.1
P1,P4-bis(5'-adenosyl) tetraphosphate
stimulation at low concentrations, inhibition above 0.3 mM
Vaccinia virus
Cloned(Commentary)
EC Number
Commentary
Organism
1.17.4.1
expression in Escherichia coli
Vaccinia virus
General Stability
EC Number
General Stability
Organism
1.17.4.1
quite susceptible to denaturation
Vaccinia virus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.4.1
Mg2+
2fold activation
Vaccinia virus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.4.1
Vaccinia virus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.4.1
ammonium sulfate, Blue Sepharose, affinity resin
Vaccinia virus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.17.4.1
0.122
-
recombinant enzyme
Vaccinia virus
Storage Stability
EC Number
Storage Stability
Organism
1.17.4.1
-80░C, several months, no loss of activity
Vaccinia virus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
dithiothreitol serves as in vitro electron donor, maximal activity with 50-75 mM
437974
Vaccinia virus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437974
Vaccinia virus
ir
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.1
8
8.8
-
Vaccinia virus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.17.4.1
6.8
8.8
60% activity at pH 7.2
Vaccinia virus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
adenyl-5'-yl-imidodiphosphate
stimulation at low concentration, inhibition above 0.3 mM
Vaccinia virus
1.17.4.1
ATP
most effective activator
Vaccinia virus
1.17.4.1
P1,P4-bis(5'-adenosyl) tetraphosphate
stimulation at low concentrations, inhibition above 0.3 mM
Vaccinia virus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.4.1
expression in Escherichia coli
Vaccinia virus
General Stability (protein specific)
EC Number
General Stability
Organism
1.17.4.1
quite susceptible to denaturation
Vaccinia virus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.4.1
Mg2+
2fold activation
Vaccinia virus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.4.1
ammonium sulfate, Blue Sepharose, affinity resin
Vaccinia virus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.17.4.1
0.122
-
recombinant enzyme
Vaccinia virus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.17.4.1
-80░C, several months, no loss of activity
Vaccinia virus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
dithiothreitol serves as in vitro electron donor, maximal activity with 50-75 mM
437974
Vaccinia virus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437974
Vaccinia virus
ir
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.1
8
8.8
-
Vaccinia virus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.17.4.1
6.8
8.8
60% activity at pH 7.2
Vaccinia virus