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Literature summary extracted from

  • Slabaugh, M.B.; Davis, R.E.; Roseman, N.A.; Mathews, C.K.
    Vaccinia virus ribonucleotide reductase expression and isolation of the recombinant large subunit (1993), J. Biol. Chem., 268, 17803-17810.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 adenyl-5'-yl-imidodiphosphate stimulation at low concentration, inhibition above 0.3 mM Vaccinia virus
1.17.4.1 ATP most effective activator Vaccinia virus
1.17.4.1 P1,P4-bis(5'-adenosyl) tetraphosphate stimulation at low concentrations, inhibition above 0.3 mM Vaccinia virus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.1 expression in Escherichia coli Vaccinia virus

General Stability

EC Number General Stability Organism
1.17.4.1 quite susceptible to denaturation Vaccinia virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Mg2+ 2fold activation Vaccinia virus

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Vaccinia virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 ammonium sulfate, Blue Sepharose, affinity resin Vaccinia virus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.1 0.122
-
recombinant enzyme Vaccinia virus

Storage Stability

EC Number Storage Stability Organism
1.17.4.1 -80°C, several months, no loss of activity Vaccinia virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin dithiothreitol serves as in vitro electron donor, maximal activity with 50-75 mM Vaccinia virus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 8 8.8
-
Vaccinia virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.4.1 6.8 8.8 60% activity at pH 7.2 Vaccinia virus