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Literature summary extracted from

  • Youdale, T.; MacManus, J.P.; Whitefield, J.F.
    Rat liver ribonucleotide reductase: separation, purification, and properties of two nonidentical subunits (1982), Can. J. Biochem., 60, 463-470.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.1 0.09
-
CDP subunit L1 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Iron 120000 Da L2 subunit of regenerating liver contains iron Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.1 45000
-
4 * 45000 + 1 * 45000 + 1 * 75000, regenerating liver Rattus norvegicus
1.17.4.1 45000
-
x * 45000 + 1 * 75000 + 1 * 45000, holoenzyme may have an alpha4,beta,beta structure, SDS-PAGE Rattus norvegicus
1.17.4.1 75000
-
4 * 45000 + 1 * 45000 + 1 * 75000, regenerating liver Rattus norvegicus
1.17.4.1 75000
-
x * 45000 + 1 * 75000 + 1 * 45000, holoenzyme may have an alpha4,beta,beta structure, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 regenerating liver, dATP-Sepharose affinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.4.1 liver regenerating liver Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.1 0.0131
-
purified subunit L2 assayed in the presenc of L1 Rattus norvegicus
1.17.4.1 0.015
-
purified subunit L1 assayed in the presenc of L2, specific for CDP Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.17.4.1 -80°C, 0.25 M sucrose, 100 mM Tris-HCl, pH 7.6, 10 mM MgCl2, 2 mM dithiothreitol, 1 month, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
Rattus norvegicus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
ir

Subunits

EC Number Subunits Comment Organism
1.17.4.1 ? x * 45000 + 1 * 75000 + 1 * 45000, holoenzyme may have an alpha4,beta,beta' structure, SDS-PAGE Rattus norvegicus
1.17.4.1 hexamer 4 * 45000 + 1 * 45000 + 1 * 75000, regenerating liver Rattus norvegicus