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Literature summary extracted from

  • Hofmann, R.; Feller, W.; Pries, M.; Follmann, H.
    Deoxyribonucleotide biosynthesis in green algae. Purification and characterization of ribonucleotide-diphosphate reductase from Scenedesmus obliquus (1985), Biochim. Biophys. Acta, 832, 98-112.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 dithiothreitol high concentrations serve as in vitro hydrogen donor Tetradesmus obliquus
1.17.4.1 additional information stimulation by effector nucleotides Tetradesmus obliquus
1.17.4.1 additional information stimulation with various substrates Tetradesmus obliquus
1.17.4.1 phosphate up to 50 mM, pH 6.7: necessary for activity, decrease of activity at higher values Tetradesmus obliquus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 1,10-phenanthroline 0.2 mM, 50% inhibition Tetradesmus obliquus
1.17.4.1 aurintricarboxylate 0.005 mM, 50% inhibition; oligomeric form Tetradesmus obliquus
1.17.4.1 dATP
-
 Tetradesmus obliquus
1.17.4.1 EDTA 10 mM, 50% inhibition Tetradesmus obliquus
1.17.4.1 Fe2+ 0.2 mM, 50% inhibition Tetradesmus obliquus
1.17.4.1 Hydroxyurea 1.5 mM, 50% inhibition Tetradesmus obliquus
1.17.4.1 Mg2+ 2 mM, 50% inhibition Tetradesmus obliquus
1.17.4.1 Mn2+ 0.2 mM, 50% inhibition Tetradesmus obliquus
1.17.4.1 N-Hydroxy-alpha-aminoheptanoate 5 mM, 50% inhibition Tetradesmus obliquus
1.17.4.1 N-Hydroxy-alpha-aminohexanoate 15 mM, 50% inhibition Tetradesmus obliquus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.1 75000
-
 2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE Tetradesmus obliquus
1.17.4.1 90000
-
 2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE Tetradesmus obliquus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin Tetradesmus obliquus thioredoxin is the physiological reductant 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Tetradesmus obliquus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1
-
 Tetradesmus obliquus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin high concentration of dithiothreitol serves as in vitro hydrogen donor, thioredoxin B of Scenedesmus obliqus and yeast thioredoxin are most effective donors Tetradesmus obliquus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin thioredoxin is the physiological reductant Tetradesmus obliquus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.4.1 ? 2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE Tetradesmus obliquus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.1 25 30
-
 Tetradesmus obliquus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.17.4.1 15 42 15°C: approx. 27% activity, 42°C: approx. 10% activity Tetradesmus obliquus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 6.5 7
-
 Tetradesmus obliquus