BRENDA - Enzyme Database

Deoxyribonucleotide biosynthesis in green algae. Purification and characterization of ribonucleotide-diphosphate reductase from Scenedesmus obliquus

Hofmann, R.; Feller, W.; Pries, M.; Follmann, H.; Biochim. Biophys. Acta 832, 98-112 (1985)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
dithiothreitol
high concentrations serve as in vitro hydrogen donor
Tetradesmus obliquus
1.17.4.1
additional information
stimulation by effector nucleotides; stimulation with various substrates
Tetradesmus obliquus
1.17.4.1
phosphate
up to 50 mM, pH 6.7: necessary for activity, decrease of activity at higher values
Tetradesmus obliquus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
1,10-phenanthroline
0.2 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
aurintricarboxylate
0.005 mM, 50% inhibition; oligomeric form
Tetradesmus obliquus
1.17.4.1
dATP
-
Tetradesmus obliquus
1.17.4.1
EDTA
10 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
Fe2+
0.2 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
Hydroxyurea
1.5 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
Mg2+
2 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
Mn2+
0.2 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
N-Hydroxy-alpha-aminoheptanoate
5 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
N-Hydroxy-alpha-aminohexanoate
15 mM, 50% inhibition
Tetradesmus obliquus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.4.1
75000
-
2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE
Tetradesmus obliquus
1.17.4.1
90000
-
2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE
Tetradesmus obliquus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
Tetradesmus obliquus
thioredoxin is the physiological reductant
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
Tetradesmus obliquus
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.4.1
Tetradesmus obliquus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.4.1
-
Tetradesmus obliquus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
high concentration of dithiothreitol serves as in vitro hydrogen donor, thioredoxin B of Scenedesmus obliqus and yeast thioredoxin are most effective donors
437932
Tetradesmus obliquus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437932
Tetradesmus obliquus
ir
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
thioredoxin is the physiological reductant
437932
Tetradesmus obliquus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437932
Tetradesmus obliquus
-
Subunits
EC Number
Subunits
Commentary
Organism
1.17.4.1
?
2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE
Tetradesmus obliquus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.4.1
25
30
-
Tetradesmus obliquus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.17.4.1
15
42
15°C: approx. 27% activity, 42°C: approx. 10% activity
Tetradesmus obliquus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.1
6.5
7
-
Tetradesmus obliquus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
dithiothreitol
high concentrations serve as in vitro hydrogen donor
Tetradesmus obliquus
1.17.4.1
additional information
stimulation by effector nucleotides; stimulation with various substrates
Tetradesmus obliquus
1.17.4.1
phosphate
up to 50 mM, pH 6.7: necessary for activity, decrease of activity at higher values
Tetradesmus obliquus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
1,10-phenanthroline
0.2 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
aurintricarboxylate
0.005 mM, 50% inhibition; oligomeric form
Tetradesmus obliquus
1.17.4.1
dATP
-
Tetradesmus obliquus
1.17.4.1
EDTA
10 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
Fe2+
0.2 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
Hydroxyurea
1.5 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
Mg2+
2 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
Mn2+
0.2 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
N-Hydroxy-alpha-aminoheptanoate
5 mM, 50% inhibition
Tetradesmus obliquus
1.17.4.1
N-Hydroxy-alpha-aminohexanoate
15 mM, 50% inhibition
Tetradesmus obliquus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.4.1
75000
-
2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE
Tetradesmus obliquus
1.17.4.1
90000
-
2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE
Tetradesmus obliquus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
Tetradesmus obliquus
thioredoxin is the physiological reductant
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
Tetradesmus obliquus
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.4.1
-
Tetradesmus obliquus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
high concentration of dithiothreitol serves as in vitro hydrogen donor, thioredoxin B of Scenedesmus obliqus and yeast thioredoxin are most effective donors
437932
Tetradesmus obliquus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437932
Tetradesmus obliquus
ir
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
thioredoxin is the physiological reductant
437932
Tetradesmus obliquus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437932
Tetradesmus obliquus
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.4.1
?
2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE
Tetradesmus obliquus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.4.1
25
30
-
Tetradesmus obliquus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.17.4.1
15
42
15°C: approx. 27% activity, 42°C: approx. 10% activity
Tetradesmus obliquus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.1
6.5
7
-
Tetradesmus obliquus