BRENDA - Enzyme Database

Ribonucleotide reductase induced by herpes simplex type 1 virus. Characterization of a distinct enzyme

Averett, D.R.; Lubbers, C.; Elion, G.B.; Spector, T.; J. Biol. Chem. 258, 9831-9838 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
3,5-diamino-1H-1,2,4-triazole
2.3 mM, 50% inhibition of CDP reduction, 2.6 mM, 50% inhibition of ADP reduction
Mesocricetus auratus
1.17.4.1
ADP
-
Mesocricetus auratus
1.17.4.1
ATP
CDP reduction is inhibited by free ATP
Mesocricetus auratus
1.17.4.1
CDP
-
Mesocricetus auratus
1.17.4.1
dADP
product inhibition
Mesocricetus auratus
1.17.4.1
dATP
2.1 mM, 50% inhibition; inhibition of CDP reduction; weak inhibition of ADP reduction
Mesocricetus auratus
1.17.4.1
dCDP
product inhibition
Mesocricetus auratus
1.17.4.1
dCTP
1.2 mM, 50% inhibition of CDP reduction, 0.89 mM, 50% inhibition of ADP reduction
Mesocricetus auratus
1.17.4.1
dGDP
product inhibition
Mesocricetus auratus
1.17.4.1
dGTP
1.2 mM, 50% inhibition of CDP reduction, 0.93 mM, 50% inhibition of ADP reduction
Mesocricetus auratus
1.17.4.1
dTTP
inhibition of CDP reduction
Mesocricetus auratus
1.17.4.1
dUDP
product inhibition
Mesocricetus auratus
1.17.4.1
EDTA
-
Mesocricetus auratus
1.17.4.1
GDP
-
Mesocricetus auratus
1.17.4.1
Hydroxyurea
-
Mesocricetus auratus
1.17.4.1
Mg2+
uncomplexed Mg2+
Mesocricetus auratus
1.17.4.1
additional information
each ribonucleoside diphosphate substrate is competitively inhibited by reduction of each other substrate
Mesocricetus auratus
1.17.4.1
UDP
-
Mesocricetus auratus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.4.1
0.00065
-
CDP
-
Mesocricetus auratus
1.17.4.1
0.0012
-
GDP
-
Mesocricetus auratus
1.17.4.1
0.012
-
ADP
-
Mesocricetus auratus
1.17.4.1
0.08
-
UDP
-
Mesocricetus auratus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.4.1
Mesocricetus auratus
-
baby hamster kidney cells, enzyme induced by Herpes simplex, virus type 1, HSV-1
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.4.1
BHK-21 cell
-
Mesocricetus auratus
-
1.17.4.1
kidney
baby hamster kidney cells
Mesocricetus auratus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
-
437917
Mesocricetus auratus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437917
Mesocricetus auratus
ir
1.17.4.1
UDP + reduced thioredoxin
-
437917
Mesocricetus auratus
2'-deoxy-UDP + oxidized thioredoxin + H2O
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.1
8.1
-
-
Mesocricetus auratus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.17.4.1
0.00058
-
CDP
vs. GDP
Mesocricetus auratus
1.17.4.1
0.0006
-
GDP
vs. ADP
Mesocricetus auratus
1.17.4.1
0.0007
-
CDP
vs. ADP
Mesocricetus auratus
1.17.4.1
0.0012
-
GDP
vs. CDP
Mesocricetus auratus
1.17.4.1
0.0018
-
GDP
vs. UDP
Mesocricetus auratus
1.17.4.1
0.0019
-
CDP
vs. UDP
Mesocricetus auratus
1.17.4.1
0.0055
-
ADP
vs. GDP
Mesocricetus auratus
1.17.4.1
0.0058
-
UDP
vs. GDP
Mesocricetus auratus
1.17.4.1
0.014
-
ADP
vs. CDP
Mesocricetus auratus
1.17.4.1
0.036
-
ADP
vs. UDP
Mesocricetus auratus
1.17.4.1
0.042
-
UDP
vs. CDP
Mesocricetus auratus
1.17.4.1
0.043
-
UDP
vs. ADP
Mesocricetus auratus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
3,5-diamino-1H-1,2,4-triazole
2.3 mM, 50% inhibition of CDP reduction, 2.6 mM, 50% inhibition of ADP reduction
Mesocricetus auratus
1.17.4.1
ADP
-
Mesocricetus auratus
1.17.4.1
ATP
CDP reduction is inhibited by free ATP
Mesocricetus auratus
1.17.4.1
CDP
-
Mesocricetus auratus
1.17.4.1
dADP
product inhibition
Mesocricetus auratus
1.17.4.1
dATP
2.1 mM, 50% inhibition; inhibition of CDP reduction; weak inhibition of ADP reduction
Mesocricetus auratus
1.17.4.1
dCDP
product inhibition
Mesocricetus auratus
1.17.4.1
dCTP
1.2 mM, 50% inhibition of CDP reduction, 0.89 mM, 50% inhibition of ADP reduction
Mesocricetus auratus
1.17.4.1
dGDP
product inhibition
Mesocricetus auratus
1.17.4.1
dGTP
1.2 mM, 50% inhibition of CDP reduction, 0.93 mM, 50% inhibition of ADP reduction
Mesocricetus auratus
1.17.4.1
dTTP
inhibition of CDP reduction
Mesocricetus auratus
1.17.4.1
dUDP
product inhibition
Mesocricetus auratus
1.17.4.1
EDTA
-
Mesocricetus auratus
1.17.4.1
GDP
-
Mesocricetus auratus
1.17.4.1
Hydroxyurea
-
Mesocricetus auratus
1.17.4.1
Mg2+
uncomplexed Mg2+
Mesocricetus auratus
1.17.4.1
additional information
each ribonucleoside diphosphate substrate is competitively inhibited by reduction of each other substrate
Mesocricetus auratus
1.17.4.1
UDP
-
Mesocricetus auratus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.17.4.1
0.00058
-
CDP
vs. GDP
Mesocricetus auratus
1.17.4.1
0.0006
-
GDP
vs. ADP
Mesocricetus auratus
1.17.4.1
0.0007
-
CDP
vs. ADP
Mesocricetus auratus
1.17.4.1
0.0012
-
GDP
vs. CDP
Mesocricetus auratus
1.17.4.1
0.0018
-
GDP
vs. UDP
Mesocricetus auratus
1.17.4.1
0.0019
-
CDP
vs. UDP
Mesocricetus auratus
1.17.4.1
0.0055
-
ADP
vs. GDP
Mesocricetus auratus
1.17.4.1
0.0058
-
UDP
vs. GDP
Mesocricetus auratus
1.17.4.1
0.014
-
ADP
vs. CDP
Mesocricetus auratus
1.17.4.1
0.036
-
ADP
vs. UDP
Mesocricetus auratus
1.17.4.1
0.042
-
UDP
vs. CDP
Mesocricetus auratus
1.17.4.1
0.043
-
UDP
vs. ADP
Mesocricetus auratus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.4.1
0.00065
-
CDP
-
Mesocricetus auratus
1.17.4.1
0.0012
-
GDP
-
Mesocricetus auratus
1.17.4.1
0.012
-
ADP
-
Mesocricetus auratus
1.17.4.1
0.08
-
UDP
-
Mesocricetus auratus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.4.1
BHK-21 cell
-
Mesocricetus auratus
-
1.17.4.1
kidney
baby hamster kidney cells
Mesocricetus auratus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
-
437917
Mesocricetus auratus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437917
Mesocricetus auratus
ir
1.17.4.1
UDP + reduced thioredoxin
-
437917
Mesocricetus auratus
2'-deoxy-UDP + oxidized thioredoxin + H2O
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.1
8.1
-
-
Mesocricetus auratus