BRENDA - Enzyme Database

Specific inhibition of the subunits of ribonucleotide reductase as a new approach to combination chemotherapy

Cory, J.G.; Sato, A.; Lasater, L.; Adv. Enzyme Regul. 19, 139-150 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
1-Formylisoquinoline thiosemicarbazone
; 0.0006 mM, 81% inhibition, 0.1 mM desferal reverses inhibition
Mus musculus
1.17.4.1
2,3-Dihydro-1H-pyrazolo[2,3-a]imidazole
-
Mus musculus
1.17.4.1
3,5-diamino-1H-1,2,4-triazole
2 mM, 41% inhibition, presence of 0.1 mM desferal potentiates inhibition; trivial name guanazole
Mus musculus
1.17.4.1
4-Methyl-5-amino-1-formylisoquinoline thiosemicarbazone
; 0.0003 mM, 93% inhibition, 0.1 mM desferal reverses inhibition
Mus musculus
1.17.4.1
Hydroxyurea
2 mM, 93% inhibition, presence of 0.1 mM desferal potentiates inhibition
Mus musculus
1.17.4.1
additional information
inhibition of reductase by hydroxyurea, guanazole and pyrazolo-imidazole is potentiated by iron-chelating agents e.g. EDTA, desferrioxamine mesylate and 8-hydroxyquinoline, inhibition by 4-methyl-5-amino-1-formylisoquinoline thiosemicarbazone and 1-formylisoquinoline thiosemicarbazone is reversed by iron chelating agents
Mus musculus
1.17.4.1
Periodate-oxidized inosine
-
Mus musculus
1.17.4.1
pyrazoloimidazol
2 mM, 79% inhibition, presence of 0.1 mM desferal potentiates inhibition, inhibits the non-heme iron subunit
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.4.1
Mus musculus
-
Ehrlich ascites tumor cells
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.4.1
Ehrlich ascites carcinoma cell
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
-
437915
Mus musculus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437915
Mus musculus
ir
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
1-Formylisoquinoline thiosemicarbazone
; 0.0006 mM, 81% inhibition, 0.1 mM desferal reverses inhibition
Mus musculus
1.17.4.1
2,3-Dihydro-1H-pyrazolo[2,3-a]imidazole
-
Mus musculus
1.17.4.1
3,5-diamino-1H-1,2,4-triazole
2 mM, 41% inhibition, presence of 0.1 mM desferal potentiates inhibition; trivial name guanazole
Mus musculus
1.17.4.1
4-Methyl-5-amino-1-formylisoquinoline thiosemicarbazone
; 0.0003 mM, 93% inhibition, 0.1 mM desferal reverses inhibition
Mus musculus
1.17.4.1
Hydroxyurea
2 mM, 93% inhibition, presence of 0.1 mM desferal potentiates inhibition
Mus musculus
1.17.4.1
additional information
inhibition of reductase by hydroxyurea, guanazole and pyrazolo-imidazole is potentiated by iron-chelating agents e.g. EDTA, desferrioxamine mesylate and 8-hydroxyquinoline, inhibition by 4-methyl-5-amino-1-formylisoquinoline thiosemicarbazone and 1-formylisoquinoline thiosemicarbazone is reversed by iron chelating agents
Mus musculus
1.17.4.1
Periodate-oxidized inosine
-
Mus musculus
1.17.4.1
pyrazoloimidazol
2 mM, 79% inhibition, presence of 0.1 mM desferal potentiates inhibition, inhibits the non-heme iron subunit
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.4.1
Ehrlich ascites carcinoma cell
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
-
437915
Mus musculus
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437915
Mus musculus
ir