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Literature summary extracted from

  • Thelander, L.; Sjöberg, B.M.; Eriksson, S.
    Ribonucleoside diphosphate reductase (Escherichia coli) (1978), Methods Enzymol., 51, 227-237.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 ATP stimulation of UDP reduction Escherichia coli
1.17.4.1 ATP stimulation of CDP reduction Escherichia coli
1.17.4.1 dATP stimulation of CDP and UDP reduction Escherichia coli
1.17.4.1 dGTP stimulation of GDP reduction Escherichia coli
1.17.4.1 dGTP stimulation of ADP reduction Escherichia coli
1.17.4.1 dTTP stimulation of UDP reduction Escherichia coli
1.17.4.1 dTTP stimulation of CDP reduction Escherichia coli
1.17.4.1 dTTP stimulation of GDP reduction Escherichia coli
1.17.4.1 dTTP stimulation of ADP reduction Escherichia coli
1.17.4.1 additional information ribonucleoside effectors are exclusively bound at effector binding sites on subunit B1 controlling substrate specificity and activity Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Escherichia coli
-
 overproducing strain
-
1.17.4.1 Escherichia coli overproducing
-
 overproducing strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 chimeric R2 genes in which C-terminal sequences in Escherichia coli subunit R2 are replaced by C-terminal sequences from the mouse subunit R2 Escherichia coli
1.17.4.1 overproducing strain Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.1 0.59
-
 purified subunit B1 Escherichia coli
1.17.4.1 2.86
-
 purified subunit B2 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
 Escherichia coli 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
 Escherichia coli overproducing 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir

Subunits

EC Number Subunits Comment Organism
1.17.4.1 tetramer alpha2,beta2, 160000 Da subunit B1 and 78000 Da subunit B2, each consisting of 2 identical or similar proteins Escherichia coli