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Literature summary extracted from

  • Blakley, R.L.
    Ribonucleoside triphosphate reductase from Lactobacillus leichmannii (1978), Methods Enzymol., 51, 246-259.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.2 dATP specific activator for CTP reduction Lactobacillus leichmannii
1.17.4.2 dCTP specific activator for UTP reduction Lactobacillus leichmannii
1.17.4.2 dGTP specific activator for ATP reduction Lactobacillus leichmannii
1.17.4.2 dTTP specific activator for ITP reduction Lactobacillus leichmannii

General Stability

EC Number General Stability Organism
1.17.4.2 fairly stable under conditions of storage, losing only about 23% of its activity in 15 months Lactobacillus leichmannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.2 2'-deoxyadenosylcobalamin
-
 Lactobacillus leichmannii
1.17.4.2 3-isoadenosylcobalamin
-
 Lactobacillus leichmannii
1.17.4.2 adenosylcobalamin
-
 Lactobacillus leichmannii
1.17.4.2 isopropylideneadenosylcobalamin
-
 Lactobacillus leichmannii
1.17.4.2 nebularylcobalamin
-
 Lactobacillus leichmannii
1.17.4.2 tubercidylcobalamin
-
 Lactobacillus leichmannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.2 0.0003
-
 adenosylcobalamin presence of 1.0 mM CTP and 1 mM dATP Lactobacillus leichmannii
1.17.4.2 0.00045
-
 adenosylcobalamin presence of 0.1 mM CTP and 1 mM dATP Lactobacillus leichmannii
1.17.4.2 0.0055
-
 adenosylcobalamin presence of 2 mM CTP without activator Lactobacillus leichmannii
1.17.4.2 0.13
-
 CTP presence of 1 mM dATP Lactobacillus leichmannii
1.17.4.2 0.22
-
 ATP presence of 1 mM dGTP Lactobacillus leichmannii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.2 Cs+ activating effect Lactobacillus leichmannii
1.17.4.2 K+ activating effect Lactobacillus leichmannii
1.17.4.2 Li+ activating effect Lactobacillus leichmannii
1.17.4.2 Na+ activating effect Lactobacillus leichmannii
1.17.4.2 NH4+ activating effect Lactobacillus leichmannii
1.17.4.2 Rb+ activating effect Lactobacillus leichmannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.2 76000
-
-
 Lactobacillus leichmannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Pseudomonas sp.
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Corynebacterium sp.
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Clostridium sp.
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Rhizobium sp.
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Euglena sp.
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Astasia sp.
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.2 Astasia sp.
-
 euglenoid flagellate
-
1.17.4.2 Clostridium sp.
-
-
-
1.17.4.2 Corynebacterium sp.
-
-
-
1.17.4.2 Euglena sp.
-
 euglenoid flagellate
-
1.17.4.2 Lactobacillus leichmannii
-
 strain ATCC 4797
-
1.17.4.2 Pseudomonas sp.
-
-
-
1.17.4.2 Rhizobium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.2
-
 Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.2 2.82
-
-
 Lactobacillus leichmannii

Storage Stability

EC Number Storage Stability Organism
1.17.4.2 -100°C, may be stored as frozen paste for at least 3 months without significant loss of activity Lactobacillus leichmannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.2 ATP + reduced thioredoxin
-
 Lactobacillus leichmannii dATP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + dihydrolipoate
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + lipoate + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + dithioerythritol
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + ? + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + dithiothreitol
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + ? + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Pseudomonas sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Corynebacterium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Clostridium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Rhizobium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Euglena sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Astasia sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Pseudomonas sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Corynebacterium sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Clostridium sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Rhizobium sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Euglena sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Lactobacillus leichmannii deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Astasia sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Pseudomonas sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Corynebacterium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Clostridium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Rhizobium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Euglena sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Astasia sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
1.17.4.2 monomer
-
 Lactobacillus leichmannii

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.2 Coalpha-(aden-9-yl)-Cobeta-adenosylcobamide
-
 Lactobacillus leichmannii
1.17.4.2 Coalpha-(benzimidazolyl)-Cobeta-adenosylcobamide
-
 Lactobacillus leichmannii
1.17.4.2 coenzyme B12 adenosylcobalamin Pseudomonas sp.
1.17.4.2 coenzyme B12 adenosylcobalamin Corynebacterium sp.
1.17.4.2 coenzyme B12 adenosylcobalamin Clostridium sp.
1.17.4.2 coenzyme B12 adenosylcobalamin Rhizobium sp.
1.17.4.2 coenzyme B12 adenosylcobalamin Euglena sp.
1.17.4.2 coenzyme B12 adenosylcobalamin Lactobacillus leichmannii
1.17.4.2 coenzyme B12 adenosylcobalamin Astasia sp.