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Literature summary extracted from

  • Hamilton, F.D.
    Ribonucleotide reductase from Euglena gracilis. A 5-deoxyadenoslycobalamin-dependent enzyme (1974), J. Biol. Chem., 249, 4428-4434.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.2 5'-deoxyadenosylcobalamin
-
 Euglena gracilis
1.17.4.2 5'-deoxyadenosylcobalamin
-
 Lactobacillus leichmannii
1.17.4.2 ATP stimulates CTP reduction Euglena gracilis
1.17.4.2 dATP specific activator for CTP reduction Euglena gracilis
1.17.4.2 dATP specific activator for CTP reduction Lactobacillus leichmannii
1.17.4.2 dCTP
-
 Euglena gracilis
1.17.4.2 dCTP
-
 Lactobacillus leichmannii
1.17.4.2 dGTP
-
 Euglena gracilis
1.17.4.2 dGTP
-
 Lactobacillus leichmannii
1.17.4.2 dTTP
-
 Euglena gracilis
1.17.4.2 dTTP
-
 Lactobacillus leichmannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.2 dTTP inhibits CTP, ATP and GTP reduction, reduction of UTP is unaffected by deoxyribonucleotides Euglena gracilis
1.17.4.2 dTTP
-
 Lactobacillus leichmannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.2 0.09
-
 CTP in presence of dATP Euglena gracilis
1.17.4.2 0.15
-
 CTP in presence of dTTP Euglena gracilis
1.17.4.2 0.25
-
 ATP in presence of dTTP Euglena gracilis
1.17.4.2 0.44
-
 ATP
-
 Euglena gracilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.2 Mg2+
-
 Escherichia coli
1.17.4.2 Mg2+ can stimulate the reaction Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.2 100000
-
 4 * 100000, SDS-PAGE Euglena gracilis
1.17.4.2 140000 150000
-
 Euglena gracilis
1.17.4.2 240000 278000
-
 Escherichia coli
1.17.4.2 440000
-
 gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Escherichia coli
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Euglena gracilis
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.2 Escherichia coli
-
-
-
1.17.4.2 Euglena gracilis
-
-
-
1.17.4.2 Euglena gracilis Z
-
-
-
1.17.4.2 Lactobacillus leichmannii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.2
-
 Escherichia coli
1.17.4.2
-
 Euglena gracilis
1.17.4.2
-
 Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.2 0.167
-
-
 Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.2 ADP + reduced thioredoxin
-
 Escherichia coli dADP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ADP + reduced thioredoxin
-
 Lactobacillus leichmannii dADP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ATP + reduced thioredoxin
-
 Euglena gracilis dATP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ATP + reduced thioredoxin
-
 Lactobacillus leichmannii dATP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ATP + reduced thioredoxin
-
 Euglena gracilis Z dATP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CDP + reduced thioredoxin
-
 Escherichia coli dCDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CDP + reduced thioredoxin
-
 Lactobacillus leichmannii dCDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CTP + reduced thioredoxin
-
 Euglena gracilis dCTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CTP + reduced thioredoxin
-
 Lactobacillus leichmannii dCTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CTP + reduced thioredoxin
-
 Euglena gracilis Z dCTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 GTP + reduced thioredoxin
-
 Euglena gracilis dGTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 GTP + reduced thioredoxin
-
 Lactobacillus leichmannii dGTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 GTP + reduced thioredoxin
-
 Euglena gracilis Z dGTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Escherichia coli 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Euglena gracilis 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Euglena gracilis Z 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Escherichia coli 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Euglena gracilis 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 UTP + reduced thioredoxin
-
 Euglena gracilis dUTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 UTP + reduced thioredoxin
-
 Lactobacillus leichmannii dUTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 UTP + reduced thioredoxin
-
 Euglena gracilis Z dUTP + oxidized thioredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.4.2 tetramer 4 * 100000, SDS-PAGE Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.2 NADPH can utilize NADPH as hydrogen donor for ribonucleotide reduction Euglena gracilis