Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, A.K.; Bhan, A.; Hopper, S.; Abrams, R.; Franzen, J.S.
    Substrate and effector binding to ribonucleoside triphosphate reductase of Lactobacillus leichmannii (1974), Biochemistry, 13, 654-661.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.2 dATP stimulation of CTP reduction Lactobacillus leichmannii
1.17.4.2 dCTP stimulation of UTP reduction Lactobacillus leichmannii
1.17.4.2 dGTP stimulation of ATP reduction Lactobacillus leichmannii
1.17.4.2 dTTP stimulation of GTP reduction Lactobacillus leichmannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.2 69300
-
 enzyme in 6 M guanidine-HCl, 0.1 M dithiothreitol, equilibrium ultracentrifugation Lactobacillus leichmannii
1.17.4.2 72500
-
 native enzyme in 0.1 M glycine buffer, pH 9.1, equilibrium ultracentrifugation Lactobacillus leichmannii
1.17.4.2 72600
-
 maleated enzyme in 0.05 M phosphate buffer, equilibrium ultracentrifugation Lactobacillus leichmannii
1.17.4.2 74500
-
 enzyme in 6 M guanidine-HCl, 0.1 M dithiothreitol, heated for 20 min at 60°C, equilibrium ultracentrifugation Lactobacillus leichmannii
1.17.4.2 75300
-
 native enzyme in 0.05 M phosphate buffer, pH 7.0, sedimentation equilibrium method Lactobacillus leichmannii
1.17.4.2 76000
-
 gel filtration Lactobacillus leichmannii
1.17.4.2 78400
-
 SDS-PAGE Lactobacillus leichmannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.2 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O Lactobacillus leichmannii
-
 ribonucleoside triphosphate + reduced thioredoxin
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.2 Lactobacillus leichmannii
-
 strain ATCC 7630
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.2
-
 Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.2 16
-
-
 Lactobacillus leichmannii

Storage Stability

EC Number Storage Stability Organism
1.17.4.2 -65°C, no detectable loss of enzyme has been observed during several years of storage of packed bacteria Lactobacillus leichmannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.2 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 Lactobacillus leichmannii ribonucleoside triphosphate + reduced thioredoxin
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
1.17.4.2 monomer 1 * 78400, SDS-PAGE Lactobacillus leichmannii

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.2 coenzyme B12
-
 Lactobacillus leichmannii