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Literature summary extracted from

  • Goulian, M.; Beck, W.S.
    Purification and properties of cobamide-dependent ribonucleotide reductase from Lactobacillus leichmannii (1966), J. Biol. Chem., 241, 4233-4242.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.2 dATP
-
 Lactobacillus leichmannii
1.17.4.2 dGTP specific activator for ATP reduction Lactobacillus leichmannii
1.17.4.2 NADPH stimulates reductase activity in crude extracts Lactobacillus leichmannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.2 dTTP inhibits ATP reduction Lactobacillus leichmannii
1.17.4.2 Mg2+ strongly inhibitory in absence of ATP Lactobacillus leichmannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.2 1.7
-
 CTP
-
 Lactobacillus leichmannii
1.17.4.2 2.3
-
 ATP
-
 Lactobacillus leichmannii
1.17.4.2 9.5
-
 CTP without ATP Lactobacillus leichmannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.2 110000
-
 sedimentation equilibrium analysis Lactobacillus leichmannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii physiological hydrogen donor is unknown 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.2 Lactobacillus leichmannii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.2
-
 Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.2 576
-
-
 Lactobacillus leichmannii

Storage Stability

EC Number Storage Stability Organism
1.17.4.2 -20°C, crude extracts relatively unstable, most of the activity disappears within a few weeks Lactobacillus leichmannii
1.17.4.2 0-4°C, acetone and hydroxylapatite fractions are quite stable, at least 70-80% of their activities remain after 3 months Lactobacillus leichmannii
1.17.4.2 0-4°C, crude extracts relatively unstable, most of the activity disappears within a few weeks Lactobacillus leichmannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.2 ADP + reduced thioredoxin most active diphosphate substrate Lactobacillus leichmannii dADP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ATP + reduced thioredoxin
-
 Lactobacillus leichmannii dATP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CDP + reduced thioredoxin poorly reduced Lactobacillus leichmannii dCDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CMP + reduced thioredoxin poorly reduced Lactobacillus leichmannii dCMP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CTP + reduced thioredoxin
-
 Lactobacillus leichmannii dCTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 GDP + reduced thioredoxin poorly reduced Lactobacillus leichmannii dGDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 GTP + reduced thioredoxin
-
 Lactobacillus leichmannii dGTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O physiological hydrogen donor is unknown Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 UDP + reduced thioredoxin poorly reduced Lactobacillus leichmannii dUDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 UTP + reduced thioredoxin
-
 Lactobacillus leichmannii dUTP + oxidized thioredoxin + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.2 5,6-dimethylbenzimidazolycobamide
-
 Lactobacillus leichmannii
1.17.4.2 coenzyme B12
-
 Lactobacillus leichmannii
1.17.4.2 dihydrolipoate
-
 Lactobacillus leichmannii