EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.9.1 | 0.0073 | - |
4-Cresol | - |
Pseudomonas putida | |
1.17.9.1 | 0.0173 | - |
2,4-Xylenol | - |
Pseudomonas putida | |
1.17.9.1 | 0.0476 | - |
4-Hydroxybenzyl alcohol | - |
Pseudomonas putida | |
1.17.9.1 | 0.072 | - |
3,4-xylenol | - |
Pseudomonas putida | |
1.17.9.1 | 0.36 | - |
4-Ethylphenol | - |
Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.9.1 | 56000 | - |
2 * 56000, one subunit is a c-cytochrome and the other a flavoprotein, SDS-PAGE after incubation with SDS and mercaptoethanol | Pseudomonas putida |
1.17.9.1 | 115000 | - |
sedimentation equilibrium ultracentrifugation | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.9.1 | additional information | Pseudomonas putida | hydroxylase A is plasmid encoded and is produced constitutively, as long as the plasmid is maintained by growth on 3,5-xylenol. Hydroxylase B is induced by growth on 4-cresol | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.9.1 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.9.1 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.9.1 | 7.52 | - |
- |
Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.17.9.1 | -20°C, stable for a few days | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.9.1 | 2,4-xylenol + acceptor + H2O | i.e. 2,4-dimethylphenol | Pseudomonas putida | 3-methyl-4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
1.17.9.1 | 3,4-dimethylphenol + acceptor + H2O | - |
Pseudomonas putida | 2-methyl-4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
1.17.9.1 | 4-cresol + acceptor + H2O | acceptor: phenazine methosulfate | Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
1.17.9.1 | 4-cresol + acceptor + H2O | acceptor: 2,6-dichlorophenol-indophenol | Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
1.17.9.1 | 4-cresol + acceptor + H2O | the enzyme catalyzes both 4-cresol hydroxylation and further oxidation of the product, 4-hydroxybenzyl alcohol to 4-hydroxybenzaldehyde | Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
1.17.9.1 | 4-ethylphenol + acceptor + H2O | - |
Pseudomonas putida | 1-(4'-hydroxyphenyl)ethanol + reduced acceptor | - |
? | |
1.17.9.1 | 4-hydroxybenzyl alcohol + acceptor | - |
Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
1.17.9.1 | additional information | hydroxylase A is plasmid encoded and is produced constitutively, as long as the plasmid is maintained by growth on 3,5-xylenol. Hydroxylase B is induced by growth on 4-cresol | Pseudomonas putida | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.17.9.1 | dimer | 2 * 56000, one subunit is a c-cytochrome and the other a flavoprotein, SDS-PAGE after incubation with SDS and mercaptoethanol | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.9.1 | cytochrome c | enzyme has 2 subunits, one is a c-cytochrome and the other a flavoprotein | Pseudomonas putida | |
1.17.9.1 | cytochrome c | a flavocytochrome c | Pseudomonas putida | |
1.17.9.1 | FAD | enzyme has 2 subunits, one is a c-cytochrome and the other a flavoprotein | Pseudomonas putida | |
1.17.9.1 | FAD | a flavocytochrome c | Pseudomonas putida |