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Literature summary extracted from

  • Hopper, D.J.; Taylor, D.G.
    The purification and properties of p-cresol-(acceptor) oxidoreductase (hydroxylating), a flavocytochrome from Pseudomonas putida (1977), Biochem. J., 167, 155-162.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.9.1 0.0073
-
4-Cresol
-
Pseudomonas putida
1.17.9.1 0.0173
-
2,4-Xylenol
-
Pseudomonas putida
1.17.9.1 0.0476
-
4-Hydroxybenzyl alcohol
-
Pseudomonas putida
1.17.9.1 0.072
-
3,4-xylenol
-
Pseudomonas putida
1.17.9.1 0.36
-
4-Ethylphenol
-
Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.9.1 56000
-
2 * 56000, one subunit is a c-cytochrome and the other a flavoprotein, SDS-PAGE after incubation with SDS and mercaptoethanol Pseudomonas putida
1.17.9.1 115000
-
sedimentation equilibrium ultracentrifugation Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.9.1 additional information Pseudomonas putida hydroxylase A is plasmid encoded and is produced constitutively, as long as the plasmid is maintained by growth on 3,5-xylenol. Hydroxylase B is induced by growth on 4-cresol ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.17.9.1 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.9.1
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.9.1 7.52
-
-
Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.17.9.1 -20°C, stable for a few days Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.9.1 2,4-xylenol + acceptor + H2O i.e. 2,4-dimethylphenol Pseudomonas putida 3-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 3,4-dimethylphenol + acceptor + H2O
-
Pseudomonas putida 2-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 4-cresol + acceptor + H2O acceptor: phenazine methosulfate Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 4-cresol + acceptor + H2O acceptor: 2,6-dichlorophenol-indophenol Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 4-cresol + acceptor + H2O the enzyme catalyzes both 4-cresol hydroxylation and further oxidation of the product, 4-hydroxybenzyl alcohol to 4-hydroxybenzaldehyde Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 4-ethylphenol + acceptor + H2O
-
Pseudomonas putida 1-(4'-hydroxyphenyl)ethanol + reduced acceptor
-
?
1.17.9.1 4-hydroxybenzyl alcohol + acceptor
-
Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
?
1.17.9.1 additional information hydroxylase A is plasmid encoded and is produced constitutively, as long as the plasmid is maintained by growth on 3,5-xylenol. Hydroxylase B is induced by growth on 4-cresol Pseudomonas putida ?
-
?

Subunits

EC Number Subunits Comment Organism
1.17.9.1 dimer 2 * 56000, one subunit is a c-cytochrome and the other a flavoprotein, SDS-PAGE after incubation with SDS and mercaptoethanol Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.9.1 cytochrome c enzyme has 2 subunits, one is a c-cytochrome and the other a flavoprotein Pseudomonas putida
1.17.9.1 cytochrome c a flavocytochrome c Pseudomonas putida
1.17.9.1 FAD enzyme has 2 subunits, one is a c-cytochrome and the other a flavoprotein Pseudomonas putida
1.17.9.1 FAD a flavocytochrome c Pseudomonas putida