EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.3 | 1,10-phenanthroline | - |
Pseudomonas putida | |
1.18.1.3 | 2,2'-dipyridyl | - |
Pseudomonas putida | |
1.18.1.3 | PCMB | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.12.11 | additional information | - |
additional information | Km-values of the NADH-ferredoxinTOL reductase component: 0.0046 mM for cytochrome and 0.0105 mM for NADH | Pseudomonas putida | |
1.18.1.3 | 0.0046 | - |
ferricytochrome c | - |
Pseudomonas putida | |
1.18.1.3 | 0.0105 | - |
NADH | reaction with cytochrome c | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.12.11 | 46000 | - |
1 * 46000, NADH-ferredoxinTOL reductase component, SDS-PAGE | Pseudomonas putida |
1.14.12.11 | 46500 | - |
NADH-ferredoxinTOL reductase component, gel filtration | Pseudomonas putida |
1.18.1.3 | 46000 | - |
1 * 46000, SDS-PAGE | Pseudomonas putida |
1.18.1.3 | 46500 | - |
gel filtration | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.11 | Pseudomonas putida | - |
- |
- |
1.18.1.3 | Pseudomonas putida | - |
NAD-ferredocinTOL reductase component of toluene dioxygenase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.12.11 | NADH-ferredoxinTOL reductase component of toluene dioxygenase | Pseudomonas putida |
1.18.1.3 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.18.1.3 | 12.3 | - |
- |
Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.12.11 | -20°C, purified NADH-ferredoxinTOL reductase component is stable for two weeks | Pseudomonas putida |
1.14.12.11 | 0-4°C, purified NADH-ferredoxinTOL reductase component is stable for up to 30 h | Pseudomonas putida |
1.18.1.3 | -20°C, 3 months, little loss of activity | Pseudomonas putida |
1.18.1.3 | 0-4°C, stable for up to 30 h | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.11 | additional information | the NADH-ferredoxinTOL reductase component catalyzes the NADH-dependent reduction of 2,6-dichloroindophenol, nitroblue tetrazolium and ferricyanide. NADPH is inactive | Pseudomonas putida | ? | - |
? | |
1.14.12.11 | toluene + NADH + O2 | - |
Pseudomonas putida | (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
1.18.1.3 | 2 ferricyanide + NADH | - |
Pseudomonas putida | 2 ferrocyanide + NAD+ + H+ | - |
? | |
1.18.1.3 | 2 ferricytochrome c + NADH | - |
Pseudomonas putida | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
1.18.1.3 | ferricytochrome c + NADH | - |
Pseudomonas putida | ferrocytochrome c + NAD+ + H+ | - |
r | |
1.18.1.3 | NADH + oxidized 2,6-dichlorophenolindophenol + H+ | - |
Pseudomonas putida | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.18.1.3 | NADH + oxidized nitro blue tetrazolium + H+ | - |
Pseudomonas putida | NAD+ + reduced nitro blue tetrazolium | - |
? | |
1.18.1.3 | oxidized ferredoxin + NADH | - |
Pseudomonas putida | reduced ferredoxin + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.12.11 | monomer | 1 * 46000, NADH-ferredoxinTOL reductase component, SDS-PAGE | Pseudomonas putida |
1.18.1.3 | monomer | 1 * 46000, SDS-PAGE | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.1.3 | NAD-ferredocinTOL reductase | component of toluene dioxygenase | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.12.11 | 6.6 | 7.5 | NADH-ferredoxinTOL reductase component | Pseudomonas putida |
1.18.1.3 | 6.6 | 7.5 | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.11 | FAD | NADH-ferredoxinTOL reductase component is a flavoprotein that contains one mol of FAD per mol of enzyme. Km: 2.5 nM | Pseudomonas putida | |
1.14.12.11 | NADH | NADH-ferredoxinTOL reductase component | Pseudomonas putida | |
1.14.12.11 | NADPH | NADPH is inactive | Pseudomonas putida | |
1.18.1.3 | FAD | can bind one mol of FAD per mol of enzyme, Km: 2.5 nM | Pseudomonas putida | |
1.18.1.3 | NADH | - |
Pseudomonas putida |