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Literature summary extracted from

  • Ma, K.; Adams, M.W.W.
    NAD(P)H:rubredoxin oxidoreductase from Pyrococcus furiosus (2001), Methods Enzymol., 334, 55-62.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.4 0.005
-
NADPH
-
Pyrococcus furiosus
1.18.1.4 0.0095
-
oxidized rubredoxin 25°C Pyrococcus furiosus
1.18.1.4 0.034
-
NADH
-
Pyrococcus furiosus
1.18.1.4 0.05
-
oxidized rubredoxin 80°C Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.4 cytoplasm
-
Pyrococcus furiosus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.4 45000
-
gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.4 oxidized rubredoxin + NADPH Pyrococcus furiosus
-
reduced rubredoxin + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.4 Pyrococcus furiosus
-
DSM 3638
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.4
-
Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.4 1000
-
-
Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.4 2 ferricytochrome c + NADH cytochrome c from horse heart Pyrococcus furiosus 2 ferrocytochrome c + NAD+ + H+
-
?
1.18.1.4 2,6-dichloroindophenol + NADH
-
Pyrococcus furiosus reduced 2,6-dichloroindophenol + NAD+ + H+
-
?
1.18.1.4 5,5'-dithiobis(2-nitrobenzoic acid) + NADH
-
Pyrococcus furiosus ?
-
?
1.18.1.4 benzyl viologen + NADH
-
Pyrococcus furiosus reduced benzyl viologen + NAD+
-
?
1.18.1.4 FAD + NADH
-
Pyrococcus furiosus FADH2 + NAD+
-
?
1.18.1.4 Fe(III) citrate + NADH
-
Pyrococcus furiosus Fe(II) citrate + NAD+
-
?
1.18.1.4 FMN + NADH
-
Pyrococcus furiosus FMNH2 + NAD+
-
?
1.18.1.4 methyl viologen + NADH
-
Pyrococcus furiosus reduced methyl viologen + NAD+ + H+
-
?
1.18.1.4 oxidized rubredoxin + NADH rubredoxin is the best substrate Pyrococcus furiosus reduced rubredoxin + NAD+
-
?
1.18.1.4 oxidized rubredoxin + NADPH
-
Pyrococcus furiosus reduced rubredoxin + NADP+
-
?
1.18.1.4 oxidized rubredoxin + NADPH
-
Pyrococcus furiosus reduced rubredoxin + NADP+ + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.18.1.4 monomer 1 * 45000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.1.4 80
-
above Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.4 7 8
-
Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.4 FAD enzyme contains FAD Pyrococcus furiosus
1.18.1.4 FMN
-
Pyrococcus furiosus
1.18.1.4 NADH
-
Pyrococcus furiosus
1.18.1.4 NADPH preferred physiological electron donor Pyrococcus furiosus